NOCTURNAL PLASMA-LEVELS OF CYTOKINES IN HEALTHY-MEN
NOCTURNAL PLASMA-LEVELS OF CYTOKINES IN HEALTHY-MEN
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DOI：
10.1007/bf02190343
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发表时间：
1992-09-01
影响因子：
4.7
通讯作者：
HOLSBOER, F
中科院分区：
文献类型：
--
作者：
GUDEWILL, S;POLLMACHER, T;HOLSBOER, F
在12名健康男性志愿者中连续测量夜间细胞因子水平12小时，包括8小时的多导睡眠监测。通过酶联免疫吸附测定法以30分钟间隔测定白细胞介素-1-β（IL-1-β）、白细胞介素-6（IL-6）和肿瘤坏死因子-α（TNF-α）的血浆浓度。在一些受试者中，细胞因子根本检测不到。在其余志愿者中（IL-1-β为27%，IL-6和TNF-α分别为58%），可以测量到接近检测限（DL）的偶然值。关于IL-1-β和IL-6，在睡眠期间高于DL的血浆水平比在之前的清醒时间期间显著更频繁。与NREM或REM发作没有时间关联。在研究的12小时期间，TNF-α值高于DL随机分布。可以得出结论，在相当大比例的健康受试者中，少量的细胞因子在夜间释放。IL-1-β和IL-6的释放与睡眠时间相关，而TNF-α的释放与睡眠时间无关。夜间细胞因子释放是否反映了睡眠和宿主防御机制之间的相互作用或睡眠独立的昼夜节律性仍有待确定。
Nocturnal cytokine levels were measured serially in 12 healthy male volunteers for 12 h, including 8 h of polygraphically monitored nocturnal sleep. Plasma concentrations of interleukin-1-beta (IL-1-beta), interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-alpha) were determined in 30-min intervals by enzyme-linked immunoadsorbant assays. In some subjects cytokines were not detectable at all. In the remaining volunteers (27% for IL-1-beta, 58% for IL-6 and TNF-alpha, respectively) occasional values near to the detection limits (DL) of the assays could be measured. With respect to IL-1-beta and IL-6, plasma levels above the DL were significantly more frequent during sleep than during the preceding time of wakefulness. No temporal association with NREM or REM episodes could be shown. TNF-alpha-values above the DL were randomly distributed across the 12-h period investigated. It is concluded that in a considerable percentage of healthy subjects small amounts of cytokines are released at night. Release of IL-1-beta and IL-6 is temporally associated with sleep, whereas the release of TNF-alpha is not. It remains to be established whether nocturnal cytokine release reflects either an interaction between sleep and host defense mechanisms or a sleep-independent circadian rhythmicity.