COUPLING OF ION-PAIR LIQUID-CHROMATOGRAPHY AND THERMOSPRAY MASS-SPECTROMETRY VIA PHASE-SYSTEM SWITCHING WITH A POLYMERIC TRAPPING COLUMN
COUPLING OF ION-PAIR LIQUID-CHROMATOGRAPHY AND THERMOSPRAY MASS-SPECTROMETRY VIA PHASE-SYSTEM SWITCHING WITH A POLYMERIC TRAPPING COLUMN
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DOI：
10.1016/0021-9673(93)83065-z
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发表时间：
1993-11-12
影响因子：
4.1
通讯作者：
BRINKMAN, UAT
中科院分区：
文献类型：
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作者：
VREEKEN, RJ;GHIJSEN, RT;BRINKMAN, UAT
采用高分子材料捕集柱（PLRP-S），通过相系统切换实现离子对液相色谱-热喷雾质谱在线联用。相系统切换用于在洗脱液进入质谱仪之前从洗脱液中除去非挥发性物质。以十六烷基三甲基溴化铵为离子对试剂，对氯代苯氧酸的离子对分离进行了优化。改性剂用量、离子对试剂浓度、缓冲液浓度等参数影响离子对在捕集柱上的吸附。通过用，例如，三氟乙酸或离子对开关，例如，甲酸铵研究了解吸过程中pH值和改性剂浓度的影响。除了目标化合物分析外，还展示了组选择性分析。以莱茵河水为例，利用该系统对水中的氯代苯氧基酸进行了鉴定。
A trapping column packed with polymeric material (PLRP-S) was used to couple ion-pair liquid chromatography on-line with thermospray mass spectrometry by phase-system switching. Phase-system switching was used to remove non-volatiles from the eluent before it entered the mass spectrometer. The total analytical system was optimized for chlorinated phenoxy acids, which were separated as ion pairs with cetyltrimethylammonium bromide as ion-pair reagent. Parameters such as percentage of modifier and concentrations of ion-pairing reagent and buffer affected the sorption of the ion-pair on the trapping column. Desorption was effected by protonation of the acids with, e.g., trifluoroacetic acid or an ion-pair switch with, e.g., ammonium formate. The influence of pH and modifier concentration during desorption was examined. In addition to target-compound analysis, group-selective analysis was also demonstrated. As an example, the system was used to identify chlorinated phenoxy acids in river Rhine water.