Some characteristics of oogenesis in the female Turkish hamster subjected to modified environments.
Some characteristics of oogenesis in the female Turkish hamster subjected to modified environments.
复制标题
环境改变后雌性土耳其仓鼠卵子发生的一些特征。
DOI：
10.1002/em.2850190409
复制
发表时间：
1992
影响因子：
2.8
通讯作者：
Ducayen-Knowles,MB
中科院分区：
文献类型：
--
作者：
Jagiello,GM;Fang,JS;Hembree,WC;Ducayen-Knowles,MB
利用冬眠的雌性土耳其仓鼠（Mesocricetus brandti）进行了体内低温对卵母细胞成熟可能产生的影响的研究。这种生理模型提供了一个机会来分析卵母细胞暴露在较长时间的降低核心温度和/或光照下随后成熟到中期II的能力。详细观察核心温度、困倦/觉醒、血清雌二醇和卵巢组织学。生发囊泡、中期I和中期II细胞双核发生率平均为37.7%。与对照组相比，实验组的总染色体补体成熟到中期II没有显著变化，但在低温或光照下暴露的动物卵母细胞中检测到非整倍体。体内核心温度降低对卵母细胞染色体补体的影响验证了许多已发表的体外温度降低研究。该模型为干扰和分析哺乳动物卵母细胞发育的许多方面提供了一个良好的生理系统。
The hibernating female Turkish hamster (Mesocricetus brandti) was utilized for a study of possible in vivo effects of cold on oocyte maturation. Such a physiologic model offered an opportunity to analyze the ability of oocytes exposed to prolonged periods of reduced core temperature and/or light to subsequently mature to Metaphase II. Detailed observations of core temperatures, torpor/arousal, serum estradiol, and ovarian histology were made. An average incidence of 37.7% binuclearity was found in the germinal vesicle, metaphase I and II occytes of this species. Maturation to Metaphase II of total chromosome complements did not vary significantly in the experimental groups compared with the control, but aneuploidy was detected in the oocytes of animals exposed to reduced temperature or light. An effect of in vivo reduced core temperatures on oocyte chromosome complements validates many of the published in vitro studies with temperature reduction. The model presents an excellent physiologic system for perturbing and analyzing many aspects of mammalian oocyte development.