Regulation of gene expression in mouse trophoblast cells by interferon-gamma
Regulation of gene expression in mouse trophoblast cells by interferon-gamma
复制标题
DOI：
10.1016/j.placenta.2007.03.009
复制
发表时间：
2007-10-01
期刊：
影响因子：
3.8
通讯作者：
Bevilacqua, E.
中科院分区：
文献类型：
--
作者：
Hoshida, M. S.;Gorjao, R.;Bevilacqua, E.
我们之前已经证明干扰素- γ (ifn - γ)在培养的小鼠滋养细胞中激活吞噬并诱导一氧化氮的产生。在本研究中，我们检测了这种细胞因子对胎盘外锥体和滋养细胞基因表达的影响。在CD-I型小鼠妊娠第7.5天植入后获得胎盘外锥体，暴露于100 U/mL ifn - γ中。干扰素γ对胎盘外锥体形态、细胞增殖和死亡也有影响。利用互补DNA大阵列和半定量RT-PCR分析基因表达。干扰素γ处理没有改变胎盘外锥体形态、滋养细胞增殖或死亡。然而，利用基因阵列技术，我们观察到ifn - γ影响发育中的滋养细胞，改变mRNA的表达水平，导致35个基因上调，7个基因下调。转录因子和免疫反应相关基因的上调表明，ifn - γ参与了免疫稳态之外的过程，在胎盘发育和功能中起着重要作用。(C) 2007 Elsevier Ltd.版权所有。
We have previously shown that interferon-gamma (IFN-gamma) activates phagocytosis and induces nitric oxide production in cultured mouse trophoblast cells. In the present study we examined the effect of this cytokine on ectoplacental cone and gene expression in trophoblast cells. Ectoplacental cones were obtained during the postimplantation period on gestational day 7.5 from CD-I mice and exposed to 100 U/mL IFN-gamma. Ectoplacental cone morphology, cell proliferation and death were also determined upon IFN-gamma treatment. Complementary DNA macroarray and semiquantitative RT-PCR were used to analyze gene expression. IFN-gamma treatment did not alter ectoplacental cone morphology, trophoblast cell proliferation or death. However, using gene array technology, we observed that IFN-gamma affected the developing trophoblast, altering the level of mRNA expression, which resulted in upregulation of 35 genes and downregulation of seven others. The upregulation of transcription factors and immune response-associated genes suggests that IFN-gamma is involved in processes beyond immunological homeostasis and plays an important role in placental development and function. (C) 2007 Elsevier Ltd. All rights reserved.