ARISA analysis of ruminal bacterial community dynamics in lactating dairy cows during the feeding cycle
ARISA analysis of ruminal bacterial community dynamics in lactating dairy cows during the feeding cycle
复制标题
DOI：
10.1016/j.anaerobe.2009.07.002
复制
发表时间：
2010-04-01
期刊：
影响因子：
2.3
通讯作者：
Weimer, Paul J.
中科院分区：
文献类型：
--
作者：
Welkie, David G.;Stevenson, David M.;Weimer, Paul J.
牛瘤胃在动物的饲养周期中经历了环境条件的实质性变化，但这些变化对微生物种群的影响尚未得到系统的研究。两个奶牛饲喂混合饲料/精料日粮在12小时的时间间隔超过4个饲养周期表现出显着的变化，瘤胃pH值和挥发性脂肪酸（VFA）浓度。自动核糖体基因间间隔区分析（ARISA）的固体和液体相关的细菌种群在2，4，6，9，和12小时后，喂养收集的样品显示了高度的细菌多样性。在所有83个样品中共检测到155种不同的扩增子长度（AL），每个样品检测到11-74种。在一头奶牛中检测到相当大比例（11%）的AL，但在另一头奶牛中未检测到。仅在液相和固相中检测到的AL的比例分别为13.5%和1.9%。对应分析表明，细菌群落组成不同的奶牛之间和固相或液相之间，但总体上的固体相关的人口内和整个喂养周期的组成变化较小。这些数据支持这样一种观点，即饲喂相同饮食的奶牛在细菌群落组成上可能存在实质性差异，并且固体相关（生物膜）群落比相关的浮游生物群落表现出更大的稳定性。出版社：Elsevier Ltd
The bovine rumen undergoes substantial changes in environmental conditions during the animal's feeding cycle, but the effects of these changes on microbial populations have not been examined systematically. Two dairy cows fed a mixed forage/concentrate ration at 12 h intervals over 4 feeding cycles displayed substantial changes in ruminal pH and volatile fatty acid (VFA) concentrations. Automated ribosomal intergenic spacer analysis (ARISA) of solid- and liquid-associated bacterial populations in samples collected at 2, 4, 6, 9, and 12 h after feeding revealed a high degree of bacterial diversity. A total of 155 different amplicon lengths (ALs) were detected across all 83 samples, and 11-74 detected per sample. A substantial proportion (11%) of the ALs was detected in one cow but not in the other. The proportions of ALs that were detected only in the liquid phase or the solid phase were 13.5% and 1.9%, respectively. Correspondence analysis indicated that bacterial community composition differed between cows and between solid or liquid phases, but overall the solid-associated population displayed less change in composition within and across feeding cycles. The data support the notion that cows fed the same diets can have substantial differences in bacterial community composition, and that the solids-associated (biofilm) communities display greater stability than do associated planktonic communities. Published by Elsevier Ltd.