Morphology and anatomy of pollen grains from male-fertile and male-sterile cultivars of chestnut (Castanea sativa Mill.)
Morphology and anatomy of pollen grains from male-fertile and male-sterile cultivars of chestnut (Castanea sativa Mill.)
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DOI：
10.1080/14620316.2007.11512261
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发表时间：
2007-01
期刊：
影响因子：
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通讯作者：
C. Mert;A. Soylu
中科院分区：
文献类型：
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作者：
C. Mert;A. Soylu
摘要 使用光学显微镜以及扫描和透射电子显微镜描述了板栗（Castanea sativa Mill.）的四个雄性可育品种（“Firdola”、“Karamehmet”、“Sarıaşlama”和“Hacıömer”）和两个雄性不育品种（“Osmanogşlu”和“Vakit Kestanesi”）的花粉形态和超微结构。雄性可育和雄性不育栗品种的花粉粒都是三孔形的，生发沟几乎延伸到粒轴的全长。花粉粒有轻微网状的外壁。花粉粒长度在 13.33 – 21.30 µm 之间变化，并且在雄性不育品种中显着减小。在品种中观察到三种不同的花粉形状：扁长形、过扁长形和亚扁长形。雄性可育品种和雄性不育品种的花粉粒超微结构没有差异。花粉粒的内壁、外壁和总壁厚度（外壁+内壁）分别测定为：83.2-153.1 nm、432.8-520.0 nm和516.0-651.6 nm；品种间差异显着（P≤0.05）。雄性可育品种花粉粒离体萌发率在11%~78%之间，品种间差异显着（P≤0.05）。观察到的品种中空花粉粒的百分比在 3% 至 32% 之间。正常花粉百分率与发芽率的相关系数为r=0.898（P≤0.01）。
Summary Pollen morphology and ultrastructure are described for four male-fertile cultivars (‘Firdola’, ‘Karamehmet’, ‘Sarıașlama’, and ‘Hacıömer’) and two male-sterile cultivars (‘OsmanogȈlu’ and ‘Vakit Kestanesi’) of chestnut (Castanea sativa Mill.) using light microscopy and both scanning and transmission electron microscopy. Pollen grains of both male-fertile and male-sterile chestnut cultivars are tricolporate, the germinal furrow extending almost the full length of the grain axis. Pollen grains have a slightly reticulate exine. Pollen grain length varied from 13.33 – 21.30 µm, and decreased significantly in the male-sterile cultivars. Three different pollen shapes were observed among the cultivars: prolate, perprolate, and sub-prolate. The ultrastructure of the pollen grains did not differ between male-fertile and male-sterile cultivars. Intine, exine and total wall thickness (exine + intine) of pollen grains were determined as: 83.2 – 153.1 nm, 432.8 – 520.0 nm, and 516.0 – 651.6 nm, respectively; and variations were significant (P ≤ 0.05) among cultivars. The percentages of in vitro germination of pollen grains of male-fertile cultivars were between 11 – 78%, and the variations among cultivars were significant (P ≤ 0.05). The percentages of empty pollen grains observed among cultivars ranged from 3 – 32%. The correlation coefficient between the percentage of normal pollen and the germination rate was r = 0.898 (P ≤ 0.01).