Eicosanoid metabolism in cerebromicrovascular endothelium.
Eicosanoid metabolism in cerebromicrovascular endothelium.
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脑微血管内皮中的类二十烷酸代谢。
DOI：
10.1152/ajpcell.1988.254.1.c37
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发表时间：
1988
期刊：
影响因子：
--
通讯作者：
Hart,MN
中科院分区：
文献类型：
--
作者：
Moore,SA;Spector,AA;Hart,MN
培养的小鼠脑微血管内皮细胞主要产生前列环素和前列腺素（PG）E2时，暴露于痕量的花生四烯酸。在较高浓度的花生四烯酸盐或离子载体A23187下，它们比前列环素产生更多的PGE 2，并且另外产生PGF 2 α和少量的类花生酸与花生四烯酸盐的羟基化衍生物共迁移。生长在生物膜过滤器上的内皮细胞从顶面和底面释放甘草素;然而，底面与顶面释放的比例高达4：1。这种极性表明，脑血管内皮细胞的胰头素可以与血管壁和脑实质的相邻细胞相互作用，在那里它们可能在脑血管紧张度和神经胶质细胞功能的控制中发挥重要作用。这些类二十烷酸也由小鼠脑微血管产生，但主要的微血管产物是12-羟基二十碳四烯酸。这表明，12-羟基二十碳四烯酸可能是丰富的脑损伤后花生四烯酸释放。培养的脑内皮细胞迅速将12-羟基二十碳四烯酸转化为极性更强的代谢物，因此可以防止这种潜在有害的羟基酸的积累。
Cultured murine cerebral microvessel endothelia produce predominantly prostacyclin and prostaglandin (PG) E2 when exposed to trace amounts of arachidonic acid. At higher concentrations of arachidonate or with ionophore A23187, they produce more PGE2 than prostacyclin and additionally make PGF2 alpha and small amounts of eicosanoids comigrating with hydroxylated derivatives of arachidonate. Endothelia grown on micropore filters release prostaglandins from both apical and basal surfaces; however, the ratio of basal to apical release is as high as 4:1. This polarity suggests that cerebral endothelial prostaglandins can interact with neighboring cells of the vessel wall and brain parenchyma, where they may play important roles in the control of cerebrovascular tone and neuroglial function. These eicosanoids also are produced by mouse brain microvessels, but the major microvessel product is 12-hydroxyeicosatetraenoic acid. This suggests that 12-hydroxyeicosatetraenoic acid may be abundant in brain injuries after arachidonate release. Cultured cerebral endothelia rapidly convert 12-hydroxyeicosatetraenoic to more polar metabolites and thus may prevent the accumulation of this potentially deleterious hydroxyacid.