Increase of circulating neutrophil and platelet microparticles during acute vasculitis and hemodialysis
Increase of circulating neutrophil and platelet microparticles during acute vasculitis and hemodialysis
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DOI：
10.1038/sj.ki.5000306
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发表时间：
2006-04-01
影响因子：
19.6
通讯作者：
Halbwachs-Mecarelli, L
中科院分区：
文献类型：
--
作者：
Daniel, L;Fakhouri, F;Halbwachs-Mecarelli, L
微粒（MP）从血细胞的释放可在各种激活信号下发生。来自中性粒细胞和血小板的MP分别在全身感染性疾病和心血管疾病中被研究。在此研究了它们在常见肾病中的作用，包括血管炎和透析，这两种疾病的特征是中性粒细胞活化。对213份来自各种肾病患者的血浆样本（包括46例血管炎患者和40例血液透析患者）进行了血小板衍生（CD 66 b阳性）和血小板衍生（CD 41 a阳性）MP的流式细胞术分析。在这些患者中还测量了全血中中性粒细胞活化期间离体释放的MP。评价与临床参数和肌酐清除率的相关性。结果显示，存在于患者或健康对照血浆中的MP来自各种来源：血小板衍生的（38 +/-22%）、嗜中性粒细胞衍生的（2.8 +/-3.8%）MP、嗜中性粒细胞/血小板MP的混合聚集体（28 +/-15%）或既不来自嗜中性粒细胞也不来自血小板的（空）31 +/-20%。急性血管炎的MP水平最高，而其他肾脏疾病的MP水平没有显着变化。血液透析期间观察到显著增加。在肾衰竭患者中，MP水平与肌酐清除率之间没有相关性。总之，中性粒细胞和血小板MP水平是血管炎急性期和透析诱导炎症期间中性粒细胞活化的非特异性标志物。中性粒细胞/血小板MP的循环聚集体共表达两种细胞类型的粘附分子，因此可能被赋予炎症和凝血调节特性。
Release of microparticles (MPs) from blood cells may occur upon various activation signals. MPs from neutrophil and platelet have been studied in systemic infectious diseases and cardiovascular diseases, respectively. They are here investigated in common nephropathies including vasculitis and dialysis, two conditions characterized by neutrophil activation. Flow cytometry analysis of neutrophil-derived (CD66b-positive) and platelet-derived (CD41a-positive) MPs was performed on 213 plasma samples from patients with various nephropathies, including 46 patients with vasculitis and 40 hemodialysis patients. MPs released ex vivo, during neutrophil activation in whole blood, were also measured in these patients. Correlations with clinical parameters and creatinine clearance were evaluated. The results show that MPs present in plasma from patients or healthy controls are from various origins: platelet-derived (38 +/- 22%), neutrophil-derived (2.8 +/- 3.8%) MPs, mixed aggregates of neutrophil/platelet MPs (28 +/- 15%) or neither from neutrophil or platelet ( null) 31 +/- 20%. Acute vasculitis showed the highest level of all types of MPs, while other nephropathies did not result in significant changes of MP levels. A significant increase was observed during hemodialysis sessions. In patients with renal failure, no correlation was seen between MP levels and creatinine clearance. In conclusion, neutrophil and platelet MP levels are non-specific markers of neutrophil activation during vasculitis acute phase and dialysis-induced inflammation. Circulating aggregates of neutrophil/platelet MPs co-express adhesion molecules of both cell types and may be thus endowed with inflammation and coagulation-thus modulating properties.