Relationship between chromosome ploidy and radiosensitivity in selected tumor sublines of common origin.
Relationship between chromosome ploidy and radiosensitivity in selected tumor sublines of common origin.
复制标题
共同起源的选定肿瘤亚系中染色体倍性与放射敏感性之间的关系。
DOI：
10.1093/jnci/25.5.1041
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发表时间：
1960
期刊：
影响因子：
--
通讯作者：
U. Norman
中科院分区：
文献类型：
--
作者：
L. Révész;U. Norman
一个以超四倍体为主的亚系，ELT/stock来源于以超二倍体为主的埃利希腹水肿瘤，ELD，同种移植后的亚致死x射线照射细胞。多倍体细胞对同种移植物反应的不同抗性可能是免疫选择的结果。两个额外的亚系，一个纯超四倍体，ELT/克隆，和一个主要的超二倍体群体，ELTD，随后从ELT/stock中选择。前者是通过分离一个超四倍体克隆获得的，后者是通过在没有免疫选择的情况下携带ELT/stock进行序列传代获得的，在这种情况下，超二倍体细胞重新获得优势。ELTD亚系在细胞学上与主ELD系难以区分。在不同x射线剂量的体外照射后，所有亚群在平均孔径为0.45µ的Millipore扩散室中腹腔内生长，从而与宿主细胞直接接触分离。在接种后的不同时间间隔定量测定细胞群体的大小。所有未处理亚系的生长行为都非常相似。然而，他们对辐射的敏感度是不同的。在相同剂量的X射线照射后，超四倍体亚系比超二倍体系更早开始生长和/或达到更大的群体规模。
A predominantly hypertetraploid subline, ELT/stock was derived from a predominantly hyperdiploid Ehrlich ascites tumor, ELD, after homotransplantation of sublethally X-irradiated cells. The ploidy shift was probably the result of immunoselection based on the differential resistance of the polyploid cells against the homograft reaction. Two additional sublines, a pure hypertetraploid, ELT/clone, and a predominantly hyperdiploid population, ELTD, were subsequently selected from ELT/stock. The former was obtained by isolating a hypertetraploid clone (s), and the latter by carrying ELT/stock in serial passage in the absence of immunoselection, in which case hyperdiploid cells regain dominance. The ELTD subline was cytologically indistinguishable from the main ELD line. After irradiationin vitrowith different X-ray doses, all sublines were grown intraperitoneally within Millipore diffusion chambers with an average pore size of 0.45µ, thus isolated from direct contact with host cells. The size of cell population was determined quantitatively at various intervals after inoculation. The growth behavior of all untreated sublines was closely similar. There was a difference in their radiosensitivity, however. After the same dose of X rays, the hypertetraploid sublines started to grow earlier and/or reached a larger population size than the hyperdiploid lines.