Synovial fluid biomarkers associated with osteoarthritis severity reflect macrophage and neutrophil related inflammation
Synovial fluid biomarkers associated with osteoarthritis severity reflect macrophage and neutrophil related inflammation
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DOI：
10.1186/s13075-019-1923-x
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发表时间：
2019-06-13
影响因子：
4.9
通讯作者：
Kraus, Virginia Byers
中科院分区：
文献类型：
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作者：
Haraden, Collin A.;Huebner, Janet L.;Kraus, Virginia Byers
背景
确定炎性骨关节炎（OA）内型个体的滑液（SF）生物标志物特征。
方法
共有48个膝关节（来自25名参与者），通过基于规则的医学（Rules Based Medicine）使用高灵敏度多重免疫测定法——Myriad人类炎症图谱（R）1.0对大量滑液生物标志物进行定量分析，其中包括47种与炎症相关的不同细胞因子、趋化因子和生长因子。采用广义估计方程（GEE）的多变量回归和错误发现率（FDR）校正来评估滑液基于规则的医学（RBM）生物标志物与反映滑膜炎症的依他福肽成像评分、膝关节骨关节炎放射学严重程度（基于凯尔格伦 - 劳伦斯（KL）分级、关节间隙狭窄和骨赘评分）、膝关节症状以及与活化巨噬细胞和膝关节骨关节炎进展相关的滑液生物标志物（包括CD14和CD163（由活化巨噬细胞释放）以及弹性蛋白酶（由活化中性粒细胞释放））之间的关联。
结果
符合FDR的滑液生物标志物的显著关联
BackgroundTo identify a synovial fluid (SF) biomarker profile characteristic of individuals with an inflammatory osteoarthritis (OA) endotype.MethodsA total of 48 knees (of 25 participants) were characterized for an extensive array of SF biomarkers quantified by Rules Based Medicine using the high-sensitivity multiplex immunoassay, Myriad Human InflammationMAP (R) 1.0, which included 47 different cytokines, chemokines, and growth factors related to inflammation. Multivariable regression with generalized estimating equations (GEE) and false discovery rate (FDR) correction was used to assess associations of SF RBM biomarkers with etarfolatide imaging scores reflecting synovial inflammation; radiographic knee OA severity (based on Kellgren-Lawrence (KL) grade, joint space narrowing, and osteophyte scores); knee joint symptoms; and SF biomarkers associated with activated macrophages and knee OA progression including CD14 and CD163 (shed by activated macrophages) and elastase (shed by activated neutrophils).ResultsSignificant associations of SF biomarkers meeting FDR