Generation of an Ihh‐mKate2‐Dre knock‐in mouse line
Generation of an Ihh‐mKate2‐Dre knock‐in mouse line
复制标题
产生 Ihh–mKate2–Dre 敲入小鼠线
作者：
Rongyu Li;Yunting Cai;Huisang Lin;Lei Dong;Mingchuan Tang;Yiming Lang;Yini Qi;Yanli Peng;Bin Zhou;Guan Yang;Yan Teng;Xiao Yang
印度刺猬（Indianhedgehog，Ihh）是Hh家族的成员，在脊椎动物的发育和体内平衡中起着重要作用。为了提高我们对表达Ihh的细胞及其后代的功能的理解，我们产生了Ihh-mKate 2 tomm 20-Dreknock-in小鼠系，其可以用荧光蛋白mKate 2标记Ihh阳性细胞，并通过Dre介导的重组追踪Ihh阳性细胞及其后代。与之前的报道一致，我们通过mKate 2免疫染色验证了生长板肥大软骨细胞和卵泡颗粒细胞中的Ihh表达，同时通过Dre报告小鼠品系Rosa 26-confetti 2证实了这些细胞中的Dre活性。我们还首次发现Ihh可以标记某些细胞类型，包括视网膜神经节细胞、浦肯野细胞和胆囊上皮细胞。综上所述，Ihh ‐ mKate 2 tomm 20 ‐Dremouse是一种遗传工具，用于检查Ihh的精确表达谱，并追踪Ihh表达细胞及其后代。
Indian hedgehog (Ihh), a member of the Hh family, plays important roles in vertebrate development and homeostasis. To improve our understanding of the function of Ihh‐expressing cells and their progeny as well, we generate anIhh‐mKate2tomm20‐Dreknock‐in mouse line that can labelIhh‐positive cells with a fluorescence protein mKate2 and traceIhh‐positive cells and their progeny via Dre‐mediated recombination. Consistent with previous reports, we verifiedIhhexpression in hypertrophic chondrocytes of growth plate and granulosa cells of ovarian follicles by mKate2 immunostaining, and meanwhile confirmed Dre activity in these cells via a Dre reporter mouse lineRosa26‐confetti2. We also found, for the first time, thatIhhcan mark some cell types, including retinal ganglion cells, Purkinje cells, and gallbladder epithelial cells. Taken together, theIhh‐mKate2tomm20‐Dremouse is a genetic tool for examining the precise expression profile ofIhhand tracingIhh‐expressing cells and their progeny.