Microfluidic Assembly of Monodisperse Vesosomes as Artificial Cell Models
Microfluidic Assembly of Monodisperse Vesosomes as Artificial Cell Models
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DOI：
10.1021/jacs.6b10977
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发表时间：
2017-01-18
影响因子：
15
通讯作者：
Huck, Wilhelm T. S.
中科院分区：
文献类型：
--
作者：
Deng, Nan-Nan;Yelleswarapu, Maaruthy;Huck, Wilhelm T. S.
囊泡体是一种嵌套的脂质体结构，具有作为先进药物递送载体、生物反应器和人工细胞的高潜力。然而，迄今为止还没有报道制备具有受控尺寸的单分散囊泡体的方法。在这里，我们报告了一个多步骤的微流控策略分层组装均匀囊泡从去湿的双重乳液模板。由我们的方法提供的控制是由同心，离心和多室脂质体的形成说明。微流控途径的囊泡提供了一个特殊的平台，以建立人工细胞的例子是在体外转录的“核”脂质体和模仿真核细胞的架构。最后，我们展示了通过在双层中插入纳米孔来运输小分子穿过核包膜。
Vesosomes are nested liposomal structures with high potential as advanced drug delivery vehicles, bioreactors and artificial cells. However, to date no method has been reported to prepare monodisperse vesosomes of controlled size. Here we report on a multistep microfluidic strategy for hierarchically assembling uniform vesosomes from dewetting of double emulsion templates. The control afforded by our method is illustrated by the formation,of concentric, pericentric and multicompartment liposomes. The microfluidic route to vesosomes offers an exceptional platform to build artificial cells as exemplified by the in vitro transcription in "nucleus" liposomes and the mimicry of the architecture of eukaryotic cells. Finally, we show the transport of small molecules across the nucleic envelope via insertion of nanopores into the bilayers.