eIF5A has a function in the elongation step of translation in yeast
eIF5A has a function in the elongation step of translation in yeast
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DOI：
10.1016/j.bbrc.2009.01.148
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发表时间：
2009-03-20
影响因子：
3.1
通讯作者：
Zanelli, Cleslei F.
中科院分区：
文献类型：
--
作者：
Gregio, Ana P. B.;Cano, Veridiana P. S.;Zanelli, Cleslei F.
推定的翻译因子eIF5A是细胞活力所必需的，并且在整个进化过程中高度保守。在这里，我们描述了eIF5A突变体和翻译起始突变体（eIF4E）或翻译延伸突变体（eEF 2）之间的遗传相互作用。单突变体和双突变体的多核糖体谱分析显示，eIF5A突变减少多核糖体流失，与翻译起始突变体相反。此外，单独的eIF5A突变体的多核糖体谱与翻译延伸突变体的多核糖体谱非常相似。此外，eIF5A的缺失导致总蛋白质合成的显著减少和平均核糖体转运时间的增加。最后，我们证明，P机构的形成被抑制在eIF5A突变体，类似的翻译延伸抑制剂放线菌酮的效果。总之，这些结果不仅加强了eIF5A在翻译中的作用，而且也有力地支持了eIF5A在蛋白质合成的延伸步骤中的功能。(C)2009 Elsevier Inc. All rights reserved.
The putative translation factor eIF5A is essential for cell viability and is highly conserved throughout evolution. Here, we describe genetic interactions between an eIF5A Mutant and a translation initiation mutant (eIF4E) or a translation elongation mutant (eEF2). Polysome profile analysis of single and double mutants revealed that mutation in eIF5A reduces polysome run-off, contrarily to translation initiation mutants. Moreover, the polysome profile of an eIF5A mutant alone is very similar to that of a translation elongation mutant. Furthermore, depletion of eIF5A causes a significant decrease in total protein synthesis and an increase of the average ribosome transit time. Finally, we, demonstrate that the formation of P bodies is inhibited in an eIF5A mutant, similarly to the effect of the translation elongation inhibitor cycloheximide. taken together, these results not only reinforce a role for eIF5A in translation but also strongly support a function for eIF5A in the elongation step of protein synthesis. (C) 2009 Elsevier Inc. All rights reserved.