CD4(+) T-CELLS PLAY A SIGNIFICANT ROLE IN ADOPTIVE IMMUNITY TO CHLAMYDIA-TRACHOMATIS INFECTION OF THE MOUSE GENITAL-TRACT
CD4(+) T-CELLS PLAY A SIGNIFICANT ROLE IN ADOPTIVE IMMUNITY TO CHLAMYDIA-TRACHOMATIS INFECTION OF THE MOUSE GENITAL-TRACT
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DOI：
10.1128/iai.63.9.3302-3308.1995
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发表时间：
1995-09-01
影响因子：
3.1
通讯作者：
CALDWELL, HD
中科院分区：
文献类型：
--
作者：
SU, H;CALDWELL, HD
研究了CD4（+）和CD8（+）T细胞过继免疫小鼠抗生殖道沙眼衣原体感染的能力。在原发性生殖道感染消退后和继发性衣原体感染后，使用从小鼠中获得的脾脏CD4（+）或CD8（+）T细胞进行连续转移实验。结果表明，在初次感染消退后或二次攻击后，从小鼠中获得的供体CD4（+）T细胞，而不是CD8（+）T细胞，可有效地将显著的抗衣原体免疫转移到未感染动物的生殖道，增殖期衣原体特异性CD4（+）细胞培养上清中淋巴因子的表达通过酶联免疫吸附测定法测定在初次感染消退后和二次攻击后从小鼠获得的T细胞，体外再刺激的保护性CD4（+）T细胞分泌白细胞介素2、γ干扰素和白细胞介素6，从原发感染消退后4个月的小鼠中获得的静息CD4（+）T细胞也能够赋予幼稚小鼠显著水平的过继保护性免疫。这些发现支持了CD4（+）T细胞在生殖道衣原体感染的获得性免疫中的重要作用，并表明该模型中的保护性CD4（+）免疫应答相对较长。
The ability of CD4(+) and CD8(+) T cells to adoptively immunize mice against Chlamydia trachomatis infection of the mouse genital tract was studied. Adoptive transfer experiments were performed with splenic CD4(+) or CD8(+) T cells obtained from mice following resolution of a primary genital tract infection and after a secondary chlamydial challenge. The results show that donor CD4(+) T cells, but not CD8(+) T cells, obtained from mice following resolution of a primary infection or after secondary challenge were effective in transferring significant antichlamydial immunity to the genital tracts of naive animals, The lymphokine profiles in the culture supernatants of proliferating Chlamydia-specific CD4(+) T cells obtained from mice following resolution of a primary infection and after secondary challenge were assayed by an enzyme-linked immunoadsorbent assay, Protective CD4(+) T cells restimulated in vitro secreted interleukin 2, gamma interferon, and interleukin 6, lymphokine profiles characteristic of both Th1- and Th2-like responses, Resting CD4(+) T cells obtained from mice 4 months following resolution of a primary infection were also capable of conferring significant levels of adoptive protective immunity to naive mice. These findings support an important role for CD4(+) T cells in acquired immunity to chlamydial infection of the genital tract and indicate that protective CD4(+) immune responses in this model are relatively long lived.