PRESENCE OF ADENOSINE-DEAMINASE ON THE SURFACE OF MONONUCLEAR BLOOD-CELLS - IMMUNOCHEMICAL LOCALIZATION USING LIGHT AND ELECTRON-MICROSCOPY
PRESENCE OF ADENOSINE-DEAMINASE ON THE SURFACE OF MONONUCLEAR BLOOD-CELLS - IMMUNOCHEMICAL LOCALIZATION USING LIGHT AND ELECTRON-MICROSCOPY
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DOI：
10.1177/39.8.1856451
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发表时间：
1991-08-01
影响因子：
3.2
通讯作者：
FRANCO, R
中科院分区：
文献类型：
--
作者：
ARAN, JM;COLOMER, D;FRANCO, R
腺苷脱氨酶，这是必要的淋巴分化和功能，以前被认为是一种胞质酶。在这份报告中，我们证明，它可以被发现与淋巴细胞的质膜。通过免疫学技术，使用光学和电子显微镜，腺苷脱氨酶定位于外侧的正常淋巴细胞和单核细胞的质膜。由于酶的表达不同，这取决于检查的细胞类型，新的假说，涉及嘌呤代谢的免疫功能障碍或免疫缺陷综合征的机制可能会被考虑。
Adenosine deaminase, which is essential for lymphoid differentiation and function, has previously been considered to be a cytosolic enzyme. In this report we demonstrate that it can be found associated with the plasma membrane of lymphocytes. By means of immunological techniques using both light and electron microscopy, adenosine deaminase was localized on the external side of the plasma membrane of normal lymphocytes and monocytes. Since the enzyme expression differed depending on the type of cell examined, new hypotheses about the mechanisms involving purine metabolism in immune dysfunctions or immunodeficiency syndromes may be considered.