Evidence for the function of hyperactivated motility in sperm.
Evidence for the function of hyperactivated motility in sperm.
复制标题
精子过度活跃运动功能的证据。
DOI：
10.1095/biolreprod44.2.375
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发表时间：
1991
影响因子：
3.6
通讯作者：
Powell,RL
中科院分区：
文献类型：
--
作者：
Suarez,SS;Katz,DF;Owen,DH;Andrew,JB;Powell,RL
在授精后，哺乳动物精子经历鞭毛跳动模式的显著变化，称为过度激活。在低粘度培养基中，未过度激活的精子鞭毛产生相对对称的低振幅波，而过度激活的精子鞭毛产生不对称的跳动模式，导致不渐进运动。由于精子在女性生殖道中遇到了高粘性和粘弹性的液体，我们比较了高活性精子与非高活性精子和过渡精子在黏度增加的介质中的进展。从尾侧附睾获得的仓鼠精子在促进能化的培养基中孵育。在孵育0、3和4小时后，大多数精子分别表现出激活、过渡和超激活的运动性。在每个时间点，从孵育中取出等量的精子，并将其加入培养基中含有0、5%、10%、20%和30% Ficoll的溶液中。与激活精子（0小时）相比，含有大部分过度激活精子（4小时）的样本保持了更高的游动速度和鞭毛速度，并且能够产生更大的力来响应增加的粘性载荷。移行精子（3 h）表现出中等反应。通过20%和30% Ficoll溶液的高激活精子的路径比单独通过培养基的路径要直得多。这是首次证明过度激活可以赋予精子在输卵管中的机械优势，在输卵管中精子可能会遇到粘性输卵管液体和粘弹性积云基质。
After insemination, mammalian sperm undergo a striking change in flagellar beat pattern, termed hyperactivation. In low-viscosity culture medium, nonhyperactivated sperm flagella generate relatively symmetrical, low-amplitude waves, while hyperactivated sperm flagella generate an asymetrical beating pattern that results in nonprogressive movement. Since sperm encounter highly viscous and viscoelastic fluids in the female reproductive tract, the progress of hyperactivated sperm was compared with that of nonhyperactivated and transitional sperm in media of increasing viscosity. Hamster sperm obtained from the caudal epididymis were incubated in a medium that promotes capacitation. After 0, 3, and 4 h of incubation, the majority of the sperm exhibited, respectively, activated, transitional, and hyperactivated motility. At each of these time points, aliquots of sperm were removed from incubation and added to solutions of 0, 5%, 10%, 20%, and 30% Ficoll in medium. Samples containing mostly hyperactivated sperm (4 h) maintained higher swimming and flagellar velocities and were able to generate greater forces in response to increased viscous loading than activated sperm (0 h). Transitional sperm (3 h) showed an intermediate response. The paths of hyperactivated sperm through solutions of 20% and 30% Ficoll were considerably straighter than those made through medium alone. This is the first demonstration that hyperactivation can confer a mechanical advantage upon sperm in the oviduct where they may encounter viscous oviductal fluid and a viscoelastic cumulus matrix.