Fiber-optic nonlinear endomicroscopy with focus scanning by using shape memory alloy actuation.
Fiber-optic nonlinear endomicroscopy with focus scanning by using shape memory alloy actuation.
复制标题
使用形状记忆合金驱动进行聚焦扫描的光纤非线性内窥镜。
DOI：
10.1117/1.3523234
复制
发表时间：
2010
影响因子：
3.5
通讯作者：
Li,Xingde
中科院分区：
文献类型：
--
作者：
Wu,Yicong;Zhang,Yuying;Xi,Jiefeng;Li,Ming-Jun;Li,Xingde
首次开发出具有内置动态聚焦扫描功能的微型光纤内窥镜，用于生物样品的 3D 双光子荧光 (TPF) 成像。快速二维横向光束扫描是通过管状压电致动器使双包层光纤悬臂梁共振来实现的。通过使用极其紧凑的电动形状记忆合金 (SMA) 移动成像探头的远端来实现慢速轴向扫描。 10 毫米长的 SMA 允许 150 微米的收缩，驱动电压仅在 50 至 100 mV 之间变化。 SMA 收缩对所施加电压的响应是非线性的，但可重复且可以精确校准。利用内窥镜对吖啶黄染色的生物组织和未染色的白纸进行深度分辨成像，结果证明了基于 SMA 的扫描光纤内窥镜进行 3D 非线性光学成像的可行性。
A miniature fiber optic endomicroscope with built-in dynamic focus scanning capability is developed for the first time for 3-D two-photon fluorescence (TPF) imaging of biological samples. Fast 2-D lateral beam scanning is realized by resonantly vibrating a double-clad fiber cantilever with a tubular piezoactuator. Slow axial scanning is achieved by moving the distal end of the imaging probe with an extremely compact electrically driven shape memory alloy (SMA). The 10-mm-long SMA allows 150-μm contractions with a driving voltage varying only from 50 to 100 mV. The response of the SMA contraction with the applied voltage is nonlinear, but repeatable and can be accurately calibrated. Depth-resolved imaging of acriflavine-stained biological tissues and unstained white paper with the endomicroscope is performed, and the results demonstrate the feasibility of 3-D nonlinear optical imaging with the SMA-based scanning fiber-optic endomicroscope.