Heterogeneous initiation regions for transcription of the chicken ovomucoid gene.
Heterogeneous initiation regions for transcription of the chicken ovomucoid gene.
复制标题
鸡卵类粘蛋白基因转录的异质起始区。
DOI：
10.1093/nar/10.18.5553
复制
发表时间：
1982
影响因子：
14.9
通讯作者：
B. O’Malley
中科院分区：
文献类型：
--
作者：
E. Lai;D. Roop;M. Tsai;S. Woo;B. O’Malley
我们用S1-核酸酶图谱鉴定了雌激素刺激的鸡输卵管卵类粘蛋白RNA转录本的5'末端。大多数卵类粘蛋白RNA分子（88-89%）起始于位于“TATATAT”序列下游约30个碱基对处的主要区域。然而，至少有一个额外的起始区，占卵类粘蛋白RNA转录本的9%。它位于主要起始区上游（5 '）约80个碱基对处。DNA序列数据显示，类似于“TATA”盒的富含AT的序列也存在于该次要区域之前30个核苷酸。我们的发现提供了额外的见解真核基因的转录起始的分子机制，并表明，在体内合成的mRNA可能不需要一个绝对的网站转录起始。
We have identified the 5' termini of ovomucoid RNA transcripts isolated from estrogen stimulated chick oviduct by S1-nuclease mapping. Most of the ovomucoid RNA molecules (88-89%) are initiated at a predominant region which is positioned at about 30 basepairs downstream from a "TATATAT" sequence. However, there is at least one additional initiation region which accounts for 9% of the ovomucoid RNA transcripts. It is located at approximately 80 basepairs further upstream (5') from the major initiation region. DNA sequence data reveal that an AT-rich sequence resembling the "TATA" box is also present 30 nucleotides before this minor region. Our finding provides additional insight into the molecular mechanism involved in the transcription initiation of eucaryotic genes, and indicates that in vivo synthesis of mRNA may not require an absolute site for initiation of transcription.