Preptin derived from proinsulin-like growth factor II (proIGF-II) is secreted from pancreatic islet β-cells and enhances insulin secretion
Preptin derived from proinsulin-like growth factor II (proIGF-II) is secreted from pancreatic islet β-cells and enhances insulin secretion
复制标题
DOI：
10.1042/0264-6021:3600431
复制
发表时间：
2001-12-01
影响因子：
4.1
通讯作者：
Cooper, GJS
中科院分区：
文献类型：
--
作者：
Buchanan, CM;Phillips, ARJ;Cooper, GJS
胰岛β细胞分泌胰岛素、胰淀素和胰抑素等激素.为了进一步寻找β细胞激素，我们从分离自培养的鼠β TC 6-F7 β细胞的分泌颗粒中纯化肽.我们鉴定了一个34个氨基酸的肽（3948 Da），对应于胰岛素原样生长因子II E肽的Asp（69）-Leul（102），我们称之为“preptin”。Preptin存在于胰岛β细胞中，并经历葡萄糖介导的与胰岛素的共分泌。合成preptin以浓度依赖性和饱和方式增加葡萄糖刺激的β TC 6-F7细胞的胰岛素分泌。Preptin输注到离体灌注的大鼠胰腺中可使葡萄糖介导的胰岛素分泌的第二阶段增加30%，而抗Preptin免疫球蛋白输注可使胰岛素分泌的第一阶段和第二阶段分别减少29%和26%。这些发现表明preptin是葡萄糖介导的胰岛素分泌的生理放大器。
Pancreatic islet beta -cells secrete the hormones insulin, amylin and pancreastatin. To search for further beta -cell hormones, we purified peptides from secretory granules isolated from cultured murine beta TC6-F7 beta -cells. We identified a 34-amino-acid peptide (3948 Da), corresponding to Asp(69)-Leul(102) of the proinsulin-like growth factor II E-peptide, which we have termed 'preptin'. Preptin, is present in islet beta -cells and undergoes glucose-mediated co-secretion with insulin. Synthetic preptin increases insulin secretion from glucose-stimulated beta TC6-F7 cells in a concentration-dependent and saturable manner. Preptin infusion into the isolated, perfused rat pancreas increases the second phase of glucose-mediated insulin secretion by 30%, while antipreptin immunoglobulin infusion decreases the first and second phases of insulin secretion by 29 and 26% respectively. These findings suggest that preptin is a physiological amplifier of glucose-mediated insulin secretion.