Proteoglycan biosynthesis is required in BC3H1 myogenic cells for modulation of vascular smooth muscle alpha-actin gene expression in response to microenvironmental signals.
Proteoglycan biosynthesis is required in BC3H1 myogenic cells for modulation of vascular smooth muscle alpha-actin gene expression in response to microenvironmental signals.
复制标题
BC3H1 生肌细胞需要蛋白聚糖生物合成来调节血管平滑肌 α-肌动蛋白基因表达以响应微环境信号。
DOI：
10.1002/jcp.1041640122
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Strauch,AR
中科院分区：
文献类型：
--
作者：
Lee,SH;Yan,H;Reeser,JC;Dillman,JM;Strauch,AR
在小鼠BC 3 H1肌源性细胞的细胞分化过程中，诱导血管平滑肌（VSM）α-肌动蛋白mRNA表达与细胞表面和细胞外基质相关硫酸化蛋白聚糖的蓄积一致。使用人工B β-D-木糖苷糖胺聚糖受体抑制肌源性细胞中的蛋白聚糖生物合成，伴随着细胞表面/细胞外基质蛋白聚糖和VSM α-肌动蛋白mRNA表达的减少，同时增加游离硫酸软骨素/硫酸皮肤素和硫酸乙酰肝素糖胺聚糖向培养基中的分泌。VSM α-actin mRNA表达的最大抑制需要在成肌细胞完全融合和静止时的细胞分化早期阻断蛋白多糖的生物合成。B β-D-木糖苷对α-肌动蛋白mRNA表达的抑制作用来自转录和转录后控制点的减弱。在静止成肌细胞中，持续的蛋白聚糖生物合成是诱导VSM α-actin mRNA所必需的，以响应细胞分化允许的底物相关大分子（SAM）或暴露于能够瞬时刺激VSM α-actin基因转录的可溶性血清因子。结果表明，有效的成肌细胞分化和VSM α-actin mRNA水平的调节依赖于完整的细胞表面蛋白聚糖来传递细胞与底物组分和血清因子相互作用产生的信号。© 1995 Wiley利斯公司
Induction of vascular smooth muscle (VSM) α‐actin mRNA expression during cytodifferentiation of mouse BC3H1 myogenic cells coincides with the accumulation of cell surface‐ and extracellular matrix‐associated sulfated proteoglycans. Inhibition of proteoglycan biosynthesis in myogenic cells using an artificial b̃‐D‐xyloside glycosaminoglycan acceptor was accompained by a reduction in cell surface/extracellular matrix proteoglycans and VSM α‐actin mRNA expression while enhanciang the secretion of free chondroitin sulfate/dermatan sulfate and heparan sulfate glycosaminoglycans into the culture medium. Maximum inhibition of VSM α‐actin mRNA expression required that proteoglycan biosynthesis be blocked during the early phase of cytodifferentiation when myoblasts were fully confluent and quiescent. The inhibitory effect of b̃‐D‐xyloside on α‐actin mRNA expression resulted from attenuation at both the transcriptional and post‐transcriptional control points. Sustained proteoglycan biosynthesis was required for induction of VSM α‐actin mRNA in quiescent myoblasts in response to cytodifferentiation‐permissive, substrate‐associated macromolecules (SAM) or upon exposure to soluble serum factors capable of transiently stimulating VSM α‐actin gene transcription. The results suggested that efficient myoblast cytodifferentiation and modulation of VSM α‐actin mRNA levels depended on intact cell surface proteoglycans to convey signals generated as a consequence of cellular interaction with substrate components and serum factors. © 1995 Wiley‐Liss, Inc.