Complete genome sequence and comparative genomics of the probiotic yeast Saccharomyces boulardii.
Complete genome sequence and comparative genomics of the probiotic yeast Saccharomyces boulardii.
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DOI：
10.1038/s41598-017-00414-2
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发表时间：
2017-03-23
影响因子：
4.6
通讯作者：
Subramanian S
中科院分区：
文献类型：
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作者：
Khatri I;Tomar R;Ganesan K;Prasad GS;Subramanian S
益生菌布拉酵母菌 (Sb) 已知可有效对抗许多胃肠道疾病和抗生素相关性腹泻。为了了解 Sb 益生菌特性的分子基础，我们确定了两种 Sb 菌株（即 Biocodex 和 unique28）的完整基因组，以及在印度作为益生菌销售的其他三种 Sb 菌株的基因组草案。我们将这些基因组与 145 种酿酒酵母 (Sc) 菌株进行了比较，以了解这些酵母之间基因组水平的相似性和差异。 Sb 与其他 Sc 的显着特征是缺少 Ty 元素 Ty1、Ty3、Ty4 和相关的 LTR。然而，我们可以识别出 Sb 中完整的 Ty2 和 Ty5 元素。所有 Sb 菌株中均不存在己糖转运蛋白 HXT11 和 HXT9 以及天冬酰胺利用的基因。我们发现絮凝蛋白基因中重复周期和重复拷贝数的差异可能与 Sb 与 Sc 相比的差异粘附有关。基于核心蛋白质组的分类法将 Sb 菌株与 Sc 的葡萄酒菌株放在一起。我们发现来自 Z. bailii 的 5 个基因渗入到 Sb 菌株和 Sc 葡萄酒菌株的 IV 号染色体中。有趣的是，涉及赋予 Sb 已知益生菌特性的基因在大多数 Sc 菌株中都是保守的。
The probiotic yeast, Saccharomyces boulardii (Sb) is known to be effective against many gastrointestinal disorders and antibiotic-associated diarrhea. To understand molecular basis of probiotic-properties ascribed to Sb we determined the complete genomes of two strains of Sb i.e. Biocodex and unique28 and the draft genomes for three other Sb strains that are marketed as probiotics in India. We compared these genomes with 145 strains of S. cerevisiae (Sc) to understand genome-level similarities and differences between these yeasts. A distinctive feature of Sb from other Sc is absence of Ty elements Ty1, Ty3, Ty4 and associated LTR. However, we could identify complete Ty2 and Ty5 elements in Sb. The genes for hexose transporters HXT11 and HXT9, and asparagine-utilization are absent in all Sb strains. We find differences in repeat periods and copy numbers of repeats in flocculin genes that are likely related to the differential adhesion of Sb as compared to Sc. Core-proteome based taxonomy places Sb strains along with wine strains of Sc. We find the introgression of five genes from Z. bailii into the chromosome IV of Sb and wine strains of Sc. Intriguingly, genes involved in conferring known probiotic properties to Sb are conserved in most Sc strains.