Genomic asymmetric epigenetic modification of transposable elements is involved in gene expression regulation of allopolyploid Brassica napus.
Genomic asymmetric epigenetic modification of transposable elements is involved in gene expression regulation of allopolyploid Brassica napus.
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DOI：
10.1111/tpj.16491
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发表时间：
2023-10
期刊：
影响因子：
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通讯作者：
Yafang Xiao;Zengde Xi;Fei Wang;Jianbo Wang
中科院分区：
文献类型：
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作者：
Yafang Xiao;Zengde Xi;Fei Wang;Jianbo Wang
多倍体是常见的，具有广泛的地理分布和环境适应性。异源多倍性可导致转座因子（TE）的激活。然而，表观遗传修饰的TE在异源多倍体的建立和进化的机制仍有待探讨。本研究以甘蓝型油菜（Brassica napus，An An Cn Cn Cn）的TE为研究对象，探讨了基因组的TE特征、TE在异源多倍体化过程中的表观遗传修饰以及TE甲基化对基因表达的调控。在B。在甘蓝型油菜中，大约50%的基因组由TE组成。TE随着基因的接近而增加，尤其是DNA转座子。TE甲基化水平与基因表达呈负相关，TE甲基化水平的变化能够调控与光强和胁迫反应相关的邻近基因的表达，从而促进异源多倍体对新环境的强适应。TE可以通过RNA指导的DNA甲基化途径和组蛋白修饰协同调节。在B的基因组进化过程中，TE的表观遗传修饰水平趋于与二倍体亲本相似。油菜。An亚基因组的TE更容易被修饰，An和Cn亚基因组中TE数量和表观遗传修饰水平的不平衡可能导致亚基因组优势的建立。我们的研究分析了B中TE定位、DNA甲基化、siRNA和组蛋白修饰的特征。napus的研究结果，强调了TE表观遗传修饰在异源多倍体形成过程中的重要性，为揭示异源多倍体的形成和进化机制提供了支持。
Polyploids are common and have a wide geographical distribution and environmental adaptability. Allopolyploidy may lead to the activation of transposable elements (TE). However, the mechanism of epigenetic modification of TEs in the establishment and evolution of allopolyploids remains to be explored. We focused on the TEs of model allopolyploid Brassica napus (An An Cn Cn ), exploring the TE characteristics of the genome, epigenetic modifications of TEs during allopolyploidization, and regulation of gene expression by TE methylation. In B. napus, approximately 50% of the genome was composed of TEs. TEs increased with proximity to genes, especially DNA transposons. TE methylation levels were negatively correlated with gene expression, and changes in TE methylation levels were able to regulate the expression of neighboring genes related to responses to light intensity and stress, which promoted powerful adaptation of allopolyploids to new environments. TEs can be synergistically regulated by RNA-directed DNA methylation pathways and histone modifications. The epigenetic modification levels of TEs tended to be similar to those of the diploid parents during the genome evolution of B. napus. The TEs of the An subgenome were more likely to be modified, and the imbalance in TE number and epigenetic modification level in the An and Cn subgenomes may lead to the establishment of subgenome dominance. Our study analyzed the characteristics of TE location, DNA methylation, siRNA, and histone modification in B. napus and highlighted the importance of TE epigenetic modifications during the allopolyploidy process, providing support for revealing the mechanism of allopolyploid formation and evolution.