RNA profiling of cell-free saliva using microarray technology
RNA profiling of cell-free saliva using microarray technology
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DOI：
10.1177/154405910408300303
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发表时间：
2004-03-01
影响因子：
7.6
通讯作者：
Wong, DTW
中科院分区：
文献类型：
--
作者：
Li, Y;Zhou, X;Wong, DTW
像其他体液一样，唾液也被用来监测人体健康和疾病。本研究验证了无细胞唾液中存在信息丰富的人类mRNA的假设。如果存在唾液，mRNA可能提供潜在的生物标志物，以识别口腔和全身疾病的高危人群和患者。收集10名正常受试者未受刺激的唾液。从无细胞唾液上清中分离RNA并线性扩增。高密度寡核苷酸微阵列分析唾液mRNA。结果表明，在无细胞唾液中存在数千种人类mrna。定量PCR （Q-PCR）分析证实了我们微阵列研究中鉴定的mRNA的存在。基于正常唾液mRNA谱建立参考数据库。我们的发现提出了一种新的唾液诊断临床方法。唾液转录组诊断（STD），用于疾病诊断和正常健康监测的潜在应用。
has been used to like other bodily fluids Saliva,, monitor human health and disease. This study tests the hypothesis that informative human mRNA exists in cell-free saliva. If present salivary, mRNA may provide potential biomarkers to identify populations and patients at high risk for oral and systemic diseases. Unstimulated saliva was collected from ten normal subjects. RNA was isolated from the cell-free saliva supernatant and linearly amplified. High-density oligonucleotide microarrays were used to profile salivary mRNA. The results demonstrated that there are thousands of human mRNAs in cell-free saliva. Quantitative PCR (Q-PCR) analysis confirmed the present of mRNA identified by our microarray study. A reference database was generated based on the mRNA profiles in normal saliva. Our finding proposes a novel clinical approach to salivary diagnostics.. Salivary Transcriptome Diagnostics (STD), for potential applications in disease diagnostics as well as normal health surveillance.