The human ABCG4 gene is regulated by oxysterols and retinoids in monocyte-derived macrophages
The human ABCG4 gene is regulated by oxysterols and retinoids in monocyte-derived macrophages
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DOI：
10.1006/bbrc.2001.5756
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发表时间：
2001-10-26
影响因子：
3.1
通讯作者：
Assmann, G
中科院分区：
文献类型：
--
作者：
Engel, T;Lorkowski, S;Assmann, G
在这里，我们报告的诱导基因表达的ABCG 4，ABC转运蛋白亚家族G的成员，从人类巨噬细胞的氧化固醇和类维生素A，激动剂的核受体LXR和RXR。克隆的ABCG 4转录物大小为3.5 kb，含有一个开放阅读框，编码646个氨基酸的多肽。在结构上，推定的ABC转运蛋白由一个核苷酸结合折叠，然后是一个簇的六个跨膜结构域，因此符合组的一半大小的ABC转运蛋白。在人ABC转运蛋白亚家族G成员中，新型转运蛋白与ABCG 1显示出最高的蛋白质序列同源性和同一性（分别为84%和72%）。基因组组织的分析表明，ABCG 4基因是由至少14个外显子，延伸跨越12.6 kb的区域上的染色体11q23.3上的大小。基于其结构特征和LXR/RXR响应调节类似于细胞脂质输出蛋白ABCA 1，我们得出结论，ABCG 4可能参与巨噬细胞脂质稳态。(C)北京：科学出版社.
Here we report the induction of gene expression of ABCG4, a member of the ABC transporter subfamily G, from human macrophages by oxysterols and retinoids, agonists of the nuclear receptors LXR and RXR. The cloned ABCG4 transcript has a size of 3.5 kb and contains an open reading frame which encodes a polypeptide of 646 amino acids. Structurally, the putative ABC transporter protein consists of a nucleotide binding fold followed by a cluster of six transmembrane-spanning domains and thus conforms to the group of half-size ABC transporters. Among the human ABC transporter subfamily G members the novel transporter shows highest protein sequence homology and identity to ABCG1 (84 and 72%, respectively). Analysis of the genomic organization demonstrates that the ABCG4 gene is composed of at least 14 exons which extend across a region of 12.6 kb in size on chromosome 11q23.3. Based on its structural features and an LXR/RXR-responsive regulation similar to the cellular lipid export protein ABCA1, we conclude that ABCG4 may be involved in macrophage lipid homeostasis. (C) 2001 Academic Press.