Methodology of 1H NMR spectroscopy of the human brain at very high magnetic fields
Methodology of 1H NMR spectroscopy of the human brain at very high magnetic fields
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DOI：
10.1007/bf03166960
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发表时间：
2005-01-01
影响因子：
1
通讯作者：
Gruetter, R
中科院分区：
文献类型：
--
作者：
Tkác, I;Gruetter, R
考虑到高磁场下光谱学的特定要求和当前可用硬件的局限性，针对4 T和7 T下的人体应用重新设计并优化了具有交错外容积抑制和VAPOR（可变功率和优化弛豫延迟）水抑制的超短回波时间刺激回波采集模式（STEAM）脉冲序列。结合自动匀场，自动参数调整和数据处理，该方法提供了一个用户友好的工具，在非常高的磁场常规H-1核磁共振（NMR）光谱的人脑。介绍了一阶和二阶匀场、单次扫描平均、频率和相位校正以及涡流的影响。LCModel分析在7 T下从人脑测量的体内H-1 NMR光谱允许非侵入性地可靠定量超过15种代谢物，说明了高场NMR光谱的潜力。在4和7 T下进行的光谱研究的例子证明了所获得的光谱质量的高再现性。
An ultrashort-echo-time stimulated echo-acquisition mode (STEAM) pulse sequence with interleaved outer volume suppression and VAPOR (variable power and optimized relaxation delays) water suppression was redesigned and optimized for human applications at 4 and 7 T, taking into account the specific requirements for spectroscopy at high magnetic fields and limitations of currently available hardware. In combination with automatic shimming, automated parameter adjustments and data processing, this method provided a user-friendly tool for routine H-1 nuclear magnetic resonance (NMR) spectroscopy of the human brain at very high magnetic fields. Effects of first- and second-order shimming, single-scan averaging, frequency and phase corrections, and eddy currents were described. LCModel analysis of an in vivo H-1 NMR spectrum measured from the human brain at 7 T allowed reliable quantification of more than fifteen metabolites noninvasively, illustrating the potential of high-field NMR spectroscopy. Examples of spectroscopic studies performed at 4 and 7 T demonstrated the high reproducibility of acquired spectra quality.