Germinal center cells: antigen specificity, heavy chain class expression and evidence of memory.
Germinal center cells: antigen specificity, heavy chain class expression and evidence of memory.
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生发中心细胞：抗原特异性、重链类表达和记忆证据。
DOI：
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发表时间：
1985
影响因子：
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通讯作者：
E. Butcher
中科院分区：
文献类型：
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作者：
G. Kraal;I. Weissman;E. Butcher
生发中心是抗原刺激后淋巴组织 B 细胞区域中 B 细胞分化的离散位点。在小鼠生发中心，细胞悬浮液中的细胞可以被可靠地识别为 PNAhi B 细胞，其结合的 PNA 是其他 B 细胞和 T 细胞或浆细胞 (PNAlo) 的 10-30 倍，并且其特征还在于缺乏表面 IgD1-3。在派尔氏集结中，大多数生发中心细胞具有 IgA 同种型，而在淋巴结中，初次刺激后的主要同种型是 IgM2,3。二次刺激淋巴结后观察到表面 IgG 表达，表明生发中心在重链类别转换中发挥重要作用2。生发中心群体包含大多数抗原结合细胞2，在生发中心细胞定位的研究中，已证明它们缺乏大多数小淋巴细胞所具有的正常迁移和归巢机制4。使用双色 FACS 分析，我们研究了生发中心发育的阶段，并使用过继转移系统观察了这些细胞的记忆潜力。
Germinal centers arise as discrete sites of B-cell differentiation in B cell areas of lymphoid tissue after antigenic stimulation. In the mouse germinal center cells can be confidently identified in cell suspensions as PNAhi B cells, binding 10–30 times as much PNA as other B and T cells or plasma cells (PNAlo) and are furthermore characterized by a lack of surface IgD1–3. In Peyer’s patches the majority of germinal center cells bear the IgA isotype whereas in lymph nodes the predominant isotype after primary stimulation is IgM2,3. Surface IgG expression was observed after secondary stimulation of the lymph nodes, suggesting an important role for the germinal center in heavy chain class switching2. The germinal center population contains the majority of antigen-binding cells2 and in a study on the localization of germinal center cells it has been demonstrated that they lack normal migratory and homing mechanisms most small lymphocytes possess4. Using two color FACS analysis we studied the stages in germinal center development and looked at the memory potential of these cells using an adoptive transfer system.