OSMIOPHILIC REAGENTS - NEW CYTOCHEMICAL PRINCIPLE FOR LIGHT + ELECTRON MICROSCOPY
OSMIOPHILIC REAGENTS - NEW CYTOCHEMICAL PRINCIPLE FOR LIGHT + ELECTRON MICROSCOPY
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DOI：
10.1126/science.146.3647.1039
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发表时间：
1964-01-01
期刊：
影响因子：
56.9
通讯作者：
UENO, H
中科院分区：
文献类型：
--
作者：
HANKER, JS;BERGMAN, RA;UENO, H
通过使用含有能够与 OSO4 选择性反应的基团的试剂，已经改变了既定的组织化学程序。这使得可以用光学和电子显微镜对特定的酶或大分子的官能团进行细胞化学演示。迄今为止使用的亲锇基团是巯基、硫代氨基甲酰基和重氮硫醚。反应部位产生的“锇黑”是电子不透明的，不溶于脂质，并且当该物质嵌入丙烯酸和环氧树脂中时也不会改变。其精细的非晶特性使其能够在电子束中精确保持。
Established histo-chemical procedures have been altered by utilizing reagents which contain a group capable of reacting selectively with OSO4. This permits the cytochemical demonstration of a particular enzyme or functional group of a macromolecule with the light and electron microscope. The osmiophilic groups used thus far are mercapto, thiocarbamyl, and diazothioether. The "osmium black" produced at the site of reaction is electron opaque, is insoluble in lipids, and is not altered when the issue is embedded in acrylic and epoxy resins. Its fine amorphous character enables it to be maintained precisely in the electron beam.