Functional elucidation of MiR-34 in osteosarcoma cells and primary tumor samples
Functional elucidation of MiR-34 in osteosarcoma cells and primary tumor samples
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DOI：
10.1016/j.bbrc.2009.07.101
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发表时间：
2009-10-09
影响因子：
3.1
通讯作者：
Wang, Daping
中科院分区：
文献类型：
--
作者：
He, Chunlei;Xiong, Jianyi;Wang, Daping
miR-34被认为是直接的p53靶点，可诱导细胞凋亡、细胞周期停滞和衰老。发现miR-34与肿瘤发生相关。到目前为止，还没有关于MiR-34 s在骨肉瘤中的作用的研究。在本研究中，我们深入研究了MiR-34 s在两种骨肉瘤细胞系U2 OS（p53（+/+））和SAOS-2（p53（/））中的功能。我们发现MiR-34 s部分以p53依赖的方式影响其靶基因的表达。p53也参与了MiR-34 s诱导的细胞周期阻滞和凋亡。最后，我们检测了117例原发性骨肉瘤中MiR-34基因的表达、遗传学和表观遗传学改变。肿瘤样本中MiR-34 s的表达减少，骨肉瘤中MiR-34基因发生了最小程度的缺失和表观遗传失活。(C)2009 Elsevier Inc. All rights reserved.
MiR-34s have been characterized as direct p53 targets, which induce apoptosis, cell cycle arrest, and senescence. MiR-34s were found to associate with tumorigenesis. Thus far, there is no study on the role of MiR-34s in osteosarcoma. In the current study, we intensively investigated the function of MiR-34s in two osteosarcoma cell lines: U2OS (p53(+/+)) and SAOS-2 (p53 (/) ). We found that MiR-34s affect the expression of its target genes partially in a p53-dependent manner. And p53 also partially contributes to the MiR-34s induced cell cycle arrest and apoptosis. Finally, we examined the expression, genetic and epigenetic alterations of MiR-34 gene in 117 primary osteosarcoma samples. Expression of MiR-34s was decreased in tumor samples, and MiR-34 genes underwent minimal deletions and epigenetic inactivation in osteosarcomas. (C) 2009 Elsevier Inc. All rights reserved.