Megasphaera lornae sp. nov., Megasphaera hutchinsoni sp. nov., and Megasphaera vaginalis sp. nov.: novel bacteria isolated from the female genital tract.
Megasphaera lornae sp. nov., Megasphaera hutchinsoni sp. nov., and Megasphaera vaginalis sp. nov.: novel bacteria isolated from the female genital tract.
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洛奈巨球菌
DOI：
10.1099/ijsem.0.004702
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发表时间：
2019
影响因子：
2.8
通讯作者：
Hillier,SharonL
中科院分区：
文献类型：
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作者：
Srinivasan,Sujatha;Beamer,MayA;Fiedler,TinaL;Austin,MicheleN;Sizova,MariaV;Strenk,SusanM;Agnew,KathyJ;Gowda,GANagana;Raftery,Daniel;Epstein,SlavaS;Fredricks,DavidN;Hillier,SharonL
从女性生殖道分离出3种严格厌氧革兰氏阴性菌6株。各菌株的预测型菌株分别为UPII 199-6T、ka00182t和BV3C16-1T。基于16S rRNA基因测序的系统发育分析表明，分离的细菌属于megasphaera属。UPII 199- 6and KA00182Thad 16S rRNA基因序列与先前从人类阴道扩增的16S rRNA克隆序列的一致性为99.9%，命名为megasphaertype 1和megasphaertype 2，这是与细菌性阴道病、早产和HIV感染相关的人类阴道微生物群成员。UPII 199-6与有效命名的megasphaerae分离株和KA00182Thad 16S rRNA基因序列的同源性在92.9 - 93.6%之间，同源性在92.6 - 94.2%之间。bv3c16 - 1t基因序列与cerevisiaegasphaera 16S rRNA基因序列的同源性为95.4%。细胞呈球型或双球型，不运动，不形成孢子。生殖道分离株代谢有机酸，但不溶糖。分离株也代谢氨基酸。UPII 199-6T、ka00182t和bv3c16 - 1t基因组序列的DNA G+C含量分别为46.4%、38.9%和49.8% mol%。数字DNA-DNA杂交和其他有效命名的megasphaeria种之间的平均核苷酸鉴定表明，每种分离类型都代表一个新物种。主要脂肪酸甲酯包括：UPII 199-6T中的C12: 0、C16: 0、C16: 0缩二甲酯（DMA）和总结特征5 (C15: 0DMA和/或C14: 03-OH)；在KA00182T中C16: 0和C16: 1cis9；c12: 0；[14:03 .噢；总结了BV3C16-1T的特性5。分离菌株产生丁酸、异丁酸和异戊酸，但在甲酸和丙酸的产生方面存在特殊差异。综上所述，UPII 199-6T、ka00182t和bv3c16 -1代表了megasphaera属的新种。我们建议将其命名为：Megasphaera lornaesp；11 . for UPII 199-6代表该物种的类型菌株（=DSM 111201T=ATCC TSD-205T），Megasphaera hutchinsonisp。11 .为ka00182代表该物种的类型菌株（=DSM 111202T=ATCC TSD-206T）和阴道巨斑菌。11 .为bv3c16 -1代表该物种的型菌株（=DSM 111203T=ATCC TSD-207T）。
Six strictly anaerobic Gram-negative bacteria representing three novel species were isolated from the female reproductive tract. The proposed type strains for each species were designated UPII 199-6T, KA00182Tand BV3C16-1T. Phylogenetic analyses based on 16S rRNA gene sequencing indicated that the bacterial isolates were members of the genusMegasphaera. UPII 199-6Tand KA00182Thad 16S rRNA gene sequence identities of 99.9 % with 16S rRNA clone sequences previously amplified from the human vagina designated asMegasphaeratype 1 andMegasphaeratype 2, members of the human vaginal microbiota associated with bacterial vaginosis, preterm birth and HIV acquisition. UPII 199-6Texhibited sequence identities ranging from 92.9 to 93.6 % with validly namedMegasphaeraisolates and KA00182Thad 16S rRNA gene sequence identities ranging from 92.6–94.2 %. BV3C16-1Twas most closely related toMegasphaera cerevisiaewith a 16S rRNA gene sequence identity of 95.4 %. Cells were coccoid or diplococcoid, non-motile and did not form spores. Genital tract isolates metabolized organic acids but were asaccharolytic. The isolates also metabolized amino acids. The DNA G+C content for the genome sequences of UPII 199-6T, KA00182Tand BV3C16-1Twere 46.4, 38.9 and 49.8 mol%, respectively. Digital DNA–DNA hybridization and average nucleotide identity between the genital tract isolates and other validly namedMegasphaeraspecies suggest that each isolate type represents a new species. The major fatty acid methyl esters include the following: C12 : 0, C16 : 0, C16 : 0dimethyl acetal (DMA) and summed feature 5 (C15 : 0DMA and/or C14 : 03-OH) in UPII 199-6T; C16 : 0and C16 : 1cis9 in KA00182T; C12 : 0; C14 : 03-OH; and summed feature 5 in BV3C16-1T. The isolates produced butyrate, isobutyrate, and isovalerate but there were specific differences including production of formate and propionate. Together, these data indicate that UPII 199-6T, KA00182Tand BV3C16-1Trepresent novel species within the genusMegasphaera. We propose the following names:Megasphaera lornaesp. nov. for UPII 199-6Trepresenting the type strain of this species (=DSM 111201T=ATCC TSD-205T),Megasphaera hutchinsonisp. nov. for KA00182Trepresenting the type strain of this species (=DSM 111202T=ATCC TSD-206T) andMegasphaera vaginalissp. nov. for BV3C16-1Trepresenting the type strain of this species (=DSM 111203T=ATCC TSD-207T).