CLONING AND CHARACTERIZATION OF THE ASD GENE OF SALMONELLA-TYPHIMURIUM - USE IN STABLE MAINTENANCE OF RECOMBINANT PLASMIDS IN SALMONELLA VACCINE STRAINS
CLONING AND CHARACTERIZATION OF THE ASD GENE OF SALMONELLA-TYPHIMURIUM - USE IN STABLE MAINTENANCE OF RECOMBINANT PLASMIDS IN SALMONELLA VACCINE STRAINS
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DOI：
10.1016/0378-1119(90)90464-3
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发表时间：
1990-09-28
期刊：
影响因子：
3.5
通讯作者：
CURTISS, R
中科院分区：
文献类型：
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作者：
GALAN, JE;NAKAYAMA, K;CURTISS, R
鼠伤寒沙门氏菌的asd突变体对二氨基庚二酸（DAP）具有专性需求，并将在缺乏DAP的环境中发生裂解。这允许开发平衡致死系统，用于使用含有来自变形链球菌和asd突变沙门氏菌宿主的野生型asd基因的载体在疫苗菌株中表达异源抗原[Nakayama等人，Biotechnology 6（1988）693-697]。我们从S.鼠伤寒沙门氏菌，其特征在于基因产物，并使用该基因构建Asd+表达克隆载体。此外，我们已经构建了一个asd盒和一个来自Tn 5的转座子，其允许快速修饰其他载体以供使用。asd疫苗株;鼠伤寒沙门氏菌增加了Asd+载体/Δ的通用性。质粒维持的asd宿主系统。
The asd mutants of Salmonella typhimurium have an obligate requirement for diaminopimelic acid (DAP) and will undergo lysis in environments deprived of DAP. This has allowed the development of a balanced-lethal system for the expression of heterologous antigens in vaccine strains using vectors containing the wild-type asd gene from Streptococcus mutans and asd mutant Salmonella hosts [Nakayama et al., Biotechnology 6 (1988) 693-697]. We have cloned the asd gene from S. typhimurium, characterized the gene product and used this gene to construct Asd+ expression cloning vectors. In addition we have constructed an asd cassette and a transposon derived from Tn5 that allow the rapid modification of other vectors for use .DELTA. asd vaccine strains of S. typhimurium adding versatility to the Asd+ vector/.DELTA. asd host system of plasmid maintenance.