New distributional records, taxonomy, morphology, and genetic variations of the endangered brackish-water species Lamprothamnium succinctum (Charales, Charophyceae) in Japan
New distributional records, taxonomy, morphology, and genetic variations of the endangered brackish-water species Lamprothamnium succinctum (Charales, Charophyceae) in Japan
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日本濒危咸水物种 Lamprothamnium succinctum（Charales，轮藻纲）的新分布记录、分类学、形态学和遗传变异
DOI：
10.1016/j.japb.2020.09.005
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发表时间：
2021
影响因子：
0.8
通讯作者：
H.
中科院分区：
文献类型：
--
作者：
Kato;S.;Tanaka;J.;Tanaka;N.;Yokoyama;J.;Ito;Y.;Fujiwara;Y.;Higa;A.; Kobayashi;S.;Watanabe;M. M. and Sakayama;H.
半咸水物种Lamprothamalphine succinctum（轮藻目，轮藻科）的成员广泛分布于热带至温带地区，包括东亚。在日本，L. succinctumis被列为濒危物种，并受到政府的保护，因为它只在两个地方被记录，八郎湖（秋田县）和大池池（出叶岛，德岛县），并已在前者灭绝。在这项研究中，我们确定了该种在日本的5个新地点。其菌体形态特征与该种原始描述一致，具有明显的生殖特征。此外，首次用扫描电子显微镜观察了日本产L. succinctum的卵孢子。日本标本的卵孢子表现出沟窝壁图案，这与以前的研究中所描述的一致。我们的遗传分析的基础上的DNA序列的两个叶绿体DNA标记，包括编码区和非编码区，显示，样品从大池池是可区分的，从其他日本标本，虽然他们是遗传上非常相似。
Members of the brackish-water speciesLamprothamnium succinctum(Charales, Charophyceae) are widely distributed from tropical to temperate regions, including East Asia. In Japan,L. succinctumis listed as an endangered species and is protected by the government, because it was recorded only at two localities, Lake Hachiro-gata (Akita Prefecture) and Oo-ike pond (Deba-jima Island, Tokushima prefecture), and has become extinct in the former. In this study, we identified five new localities of this species in Japan. The morphological characteristics of their thalli agreed with those provided in the original description of this species, with distinctive reproductive characteristics. Moreover, the oospores of Japanese specimens ofL. succinctumwere examined for the first time using scanning electron microscopy. The oospores of Japanese specimens exhibited granulate fossa wall patterns, which were consistent with those described in previous studies. Our genetic analyses based on the DNA sequences of two chloroplast DNA markers, including both the coding and non-coding regions, revealed that the sample from Oo-ike pond is distinguishable from those from other Japanese specimens, although they are genetically very similar.