Comparative proteome analysis of porcine follicular fluid and serum reveals that excessive α2-macroglobulin in serum hampers successful expansion of cumulus-oocyte complexes
Comparative proteome analysis of porcine follicular fluid and serum reveals that excessive α2-macroglobulin in serum hampers successful expansion of cumulus-oocyte complexes
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DOI：
10.1002/pmic.200900270
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发表时间：
2009-10-01
期刊：
影响因子：
3.4
通讯作者：
Maes, Dominiek
中科院分区：
文献类型：
--
作者：
Bijttebier, Jo;Tilleman, Kelly;Maes, Dominiek
猪卵泡液（pFF）构成了卵母细胞成熟的微环境。虽然pFF是血清的渗出液，但在猪中，它在促进卵丘细胞（卵母细胞周围的特化细胞群）的体外扩增方面上级血清。对自体血清和pFF进行比较蛋白质组分析，以研究参与成功卵丘扩张的蛋白质。猪卵母细胞。iTRAQ标记后进行2-D LC ESI-Q-TOF MS/MS显示两种液体共有63种蛋白质，其中13种蛋白质的丰度显著不同（p
Porcine follicular fluid (pFF) constitutes the micro-environment of the maturing oocyte. Although pFF is a transudate of serum, in pigs, it is superior to serum in promoting in vitro expansion of the cumulus cells, a specialized cell population surrounding the oocyte. A comparative proteome analysis of autologous serum and pFF was performed to investigate proteins involved in successful cumulus expansion. of porcine oocytes. iTRAQ labeling followed by 2-D LC ESI-Q-TOF MS/MS revealed 63 proteins common to both fluids of which the abundance of 13 proteins was significantly different (p