Chalcone synthase cosuppression phenotypes in petunia flowers: Comparison of sense vs antisense constructs and single-copy vs complex T-DNA sequences
Chalcone synthase cosuppression phenotypes in petunia flowers: Comparison of sense vs antisense constructs and single-copy vs complex T-DNA sequences
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DOI：
10.1007/bf00040715
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发表时间：
1996-08-01
影响因子：
5.1
通讯作者：
Napoli, CA
中科院分区：
文献类型：
--
作者：
Jorgensen, RA;Cluster, PD;Napoli, CA
花色素沉着模式进行了评分，在185个正义查尔酮合酶（Chs）转基因和85个反义Chs转基因，首次开花后，139（75%）的正义转基因被发现是表型改变，70（82%）的反义转基因。所观察到的模式文件的表型变异的范围内发生，以及确认和扩展的发现，正义Chs构建体产生几种类型的基于形态的花色素沉着模式，反义Chs构建体不。对44个有义Chs转基因群体的表观遗传变异的长期监测显示，43个（98%）能够产生共抑制表型。感特定模式的共同抑制的Chs的主要决定因素被认为是重复性和组织模式的转基因，而不是“位置效应”，或“通读”，侧翼植物DNA序列。在携带多个分散拷贝的转基因的后代中观察到的共抑制程度与用转基因的单个拷贝观察到的共抑制程度相比表明，Chs的有义共抑制受到转基因剂量效应的影响。
Flower pigmentation patterns were scored in 185 sense Chalcone synthase (Chs) transgenotes and 85 antisense Chs transgenotes; upon first flowering, 139 (75%) of sense transgenotes were found to be phenotypically altered, as were 70 (82%) of the antisense transgenotes. The observed patterns document the range of phenotypic variations that occur, as well as confirm and extend the finding that sense Chs constructs produce several types of morphology-based flower pigmentation patterns that antisense Chs constructs do not. Long-term monitoring for epigenetic variations in one population of 44 sense Chs transgenotes showed that 43 (98%) were capable of producing a cosuppression phenotype. The primary determinant of sense-specific patterns of cosuppression of Chs was found to be the repetitiveness and organization pattern of the transgene, not 'position effects' by, or 'readthrough' from, flanking plant DNA sequences. The degree of cosuppression observed in progeny of transgenotes carrying multiple, dispersed copies as compared to that observed with a single copy of the transgene suggests that sense cosuppression of Chs is subject to a transgene dosage effect.