Detection of Human Parechovirus in Stool Samples Collected From Children With Acute Gastroenteritis in Japan During 2007-2008
Detection of Human Parechovirus in Stool Samples Collected From Children With Acute Gastroenteritis in Japan During 2007-2008
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DOI：
10.1002/jmv.21740
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发表时间：
2011-02-01
影响因子：
12.7
通讯作者：
Ushijima, Hiroshi
中科院分区：
文献类型：
--
作者：
Ngan Thi Kim Pham;Chan-It, Wisoot;Ushijima, Hiroshi
2007年7月至2008年6月，日本5个地区（札幌、东京、舞鹤、大阪、佐贺）儿科诊所采集的患有急性胃肠炎的婴儿和儿童的477份粪便标本，经过轮状病毒、腺病毒、诺如病毒、沙波病毒和星状病毒筛查，其中247份（51.7%）人副肠病毒筛查呈阴性。使用引物对扩增其基因组的 5'UTR 区域，通过 RT-PCR 检测人副肠孤病毒 (HPeV)，并通过 VP1 基因测序进行基因分型。 247 份检测样本中，有 20 份检测出 HPeV，检出率为 8.1%。 20 株 HPeV 检测呈阳性的菌株中，有 17 株的 VP1 基因已成功测序。大多数 HPeV 菌株（n = 15）可被鉴定为 HPeV1，其余 2 株可被定型为 HPeV3。通过对HPeV VP1序列进行系统发育和同源矩阵分析，HPeV1应分为两个谱系，日本研究的所有HPeV1毒株均属于谱系2。这是日本首次报道 HPeV，尤其是 HPeV1 的流通情况。 J. Med。病毒。 83:331-336, 2011。(C) 2010 Wiley-Liss, Inc.
Of 477 stool specimens, which had been screened for rotavirus, adenovirus, norovirus, sapovirus and astrovirus, collected from infants and children with acute gastroenteritis in pediatric clinics encompassing five localities (Sapporo, Tokyo, Maizuru, Osaka, and Saga) in Japan from July 2007 to June 2008, 247 negative samples (51.7%) were subjected to screening for human parechovirus. Human parechovirus (HPeV) was detected by RT-PCR using a primer pair to amplify 5' UTR region of its genome and was genotyped by sequencing of the VP1 gene. HPeV was detected in 20 of 247 specimens tested, and the detection rate was found to be 8.1%. Seventeen of the 20 strains that tested positive for HPeV were sequenced successfully the VP1 gene. The majority of the HPeV strains (n = 15) could be identified as HPeV1, and the remaining 2 strains could be typed as HPeV3. By phylogenetic and identical matrix analyses of HPeV VP1 sequences, HPeV1 should be divided into two lineages, and all of the Japanese studied HPeV1 strains belong to the lineage 2 accordingly. This is the first report a the circulation of HPeV, especially HPeV1 in Japan. J. Med. Virol. 83:331-336, 2011. (C) 2010 Wiley-Liss, Inc.