Differences in vaginal microbiome in African American women versus women of European ancestry
Differences in vaginal microbiome in African American women versus women of European ancestry
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DOI：
10.1099/mic.0.081034-0
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发表时间：
2014-10-01
期刊：
影响因子：
2.8
通讯作者：
Buck, Gregory A.
中科院分区：
文献类型：
--
作者：
Fettweis, Jennifer M.;Brooks, J. Paul;Buck, Gregory A.
欧洲血统的女性更有可能拥有以乳酸杆菌为主的微生物组，而非洲裔美国女性更有可能表现出多样化的微生物特征。非洲裔美国妇女被诊断为细菌性阴道病的可能性也是两倍，早产的可能性也是两倍。本研究的目的是进一步表征和对比非裔美国人与欧洲血统妇女的阴道微生物谱。通过弗吉尼亚联邦大学的阴道人类微生物组项目，使用168 rRNA基因序列分析来比较来自1268名非洲裔美国妇女和416名欧洲血统妇女的阴道样本的微生物组。结果证实了两组阴道微生物组的显着差异，并确定了与这些差异相关的几个分类群。主要的社区类型占主导地位的加德纳菌阴道炎和未培养的细菌性阴道病相关细菌-1（BVAB 1），是常见的非洲裔美国人。此外，与微生物侵入羊膜腔和早产相关的多种细菌分类群（包括支原体、加德纳菌、普雷沃菌和Sneathia）的患病率在两个种族群体之间存在差异。我们调查了内在和外在因素的贡献，包括怀孕，体重指数，饮食，吸烟和饮酒，性伴侣的数量和家庭收入，阴道社区组成。种族、怀孕和饮酒与细菌性阴道病相关物种的相对丰度显著相关。观察到微生物谱与吸烟和性伴侣数量之间的趋势;然而，这些关联在统计学上并不显著。这些结果支持并扩展了先前的研究结果，即与种族相关的阴道微生物组存在显著差异，并表明即使在健康女性中，这些差异也很明显。
Women of European ancestry are more likely to harbour a Lactobacillus-dominated microbiome, whereas African American women are more likely to exhibit a diverse microbial profile. African American women are also twice as likely to be diagnosed with bacterial vaginosis and are twice as likely to experience preterm birth. The objective of this study was to further characterize and contrast the vaginal microbial profiles in African American versus European ancestry women. Through the Vaginal Human Microbiome Project at Virginia Commonwealth University, 168 rRNA gene sequence analysis was used to compare the microbiomes of vaginal samples from 1 268 African American women and 416 women of European ancestry. The results confirmed significant differences in the vaginal microbiomes of the two groups and identified several taxa relevant to these differences. Major community types were dominated by Gardnerella vaginalis and the uncultivated bacterial vaginosis-associated bacterium-1 (BVAB1) that were common among African Americans. Moreover, the prevalence of multiple bacterial taxa that are associated with microbial invasion of the amniotic cavity and preterm birth, including Mycoplasma, Gardnerella, Prevotella and Sneathia, differed between the two ethnic groups. We investigated the contributions of intrinsic and extrinsic factors, including pregnancy, body mass index, diet, smoking and alcohol use, number of sexual partners, and household income, to vaginal community composition. Ethnicity, pregnancy and alcohol use correlated significantly with the relative abundance of bacterial vaginosis-associated species. Trends between microbial profiles and smoking and number of sexual partners were observed; however, these associations were not statistically significant. These results support and extend previous findings that there are significant differences in the vaginal microbiome related to ethnicity and demonstrate that these differences are pronounced even in healthy women.