Structural studies of cell surface and secreted IgG in LPS-stimulated murine B cells.
Structural studies of cell surface and secreted IgG in LPS-stimulated murine B cells.
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LPS 刺激的鼠 B 细胞的细胞表面和分泌 IgG 的结构研究。
DOI：
10.1016/0161-5890(83)90018-4
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发表时间：
1983
影响因子：
3.6
通讯作者：
Vitetta,ES
中科院分区：
文献类型：
--
作者：
Yuan,D;Vitetta,ES
我们用表面碘化和特异性免疫沉淀法鉴定了LPS刺激的B淋巴细胞的细胞表面IgG。LPS刺激5天后，B细胞表面主要表达IgG亚类IgG 3。相反。IgG2b、IgG3和IgG1亚类均为分泌型。细胞表面IgG具有分泌型IgG所不具有的去污剂结合特性，并且所检测的表面IgG的三个亚类的γ链具有表观摩尔比。64 Kd的。比分泌的IgG的H链大约9.000。
We have characterized the cell surface IgG of LPS-stimulated B lymphocytes by surface iodination and specific immunoprecipitation. IgG3was found to be the predominant IgG subclass expressed on the B cell surface after 5 days of stimulation with LPS. In contrast. IgG2b, IgG3, and IgG1subclasses are all secreted. Cell surface IgG exhibits detergent binding characteristics not shown by secreted IgG and the y chains of the three subclasses of surface IgG examined have the apparent mol. wt of 64 Kd. some 9.000 larger than the H chains of secreted IgG.