Interaction network of ABA receptors in grey poplar
Interaction network of ABA receptors in grey poplar
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DOI：
10.1111/tpj.13646
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发表时间：
2017-10
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影响因子：
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通讯作者：
Michael Papacek;A. Christmann;E. Grill
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文献类型：
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作者：
Michael Papacek;A. Christmann;E. Grill
植物激素脱落酸 (ABA) 是应对非生物胁迫的关键因素。 ABA 通过控制气孔气体交换、水导度和差异基因表达来调节植物的水分状况并介导耐旱性。 ABA 被 ABA 受体 (RCAR)/PYR1/PYL（吡拉菌素抗性 1/PYR1 样）的调节成分识别并结合。配体结合可稳定 RCAR 与 2C 型蛋白磷酸酶 (PP2C)（ABA 共受体）的相互作用。虽然 ABA 信号传导的核心途径已被阐明，但植物物种内大量不同的 ABA 受体和共受体产生了复杂的异聚受体复合物，迄今为止在任何植物物种中尚未在功能上得到解决。在本研究中，我们表征了灰杨 (Populus x canescens [Ait.] Sm.) 的 ABA 受体和辅助受体及其调节 ABA 反应的能力。我们观察到大量全受体复合物的调节组合，但也观察到一些优先和选择性的 RCAR-PP2C 相互作用。杨树和拟南芥ABA受体成分显示出很强的结构和功能保守性。杨树和拟南芥成分的异源受体复合物在体外显示出功能性，并在两个物种的细胞中调节 ABA 响应基因的表达。拟南芥的 ABA 响应启动子在杨树中也很活跃，研究人员探索了产生响应 ABA 表达绿色荧光蛋白的杨树报告系。该研究对树种的受体复合物进行了详细分析，并显示一年生植物和多年生植物之间 ABA 受体成分的高度保守性。
The plant hormone abscisic acid (ABA) is a key player in responses to abiotic stress. ABA regulates a plant's water status and mediates drought tolerance by controlling stomatal gas exchange, water conductance and differential gene expression. ABA is recognized and bound by the Regulatory Component of ABA Receptors (RCARs)/PYR1/PYL (Pyrabactin Resistance 1/PYR1-like). Ligand binding stabilizes the interaction of RCARs with type 2C protein phosphatases (PP2C), which are ABA co-receptors. While the core pathway of ABA signalling has been elucidated, the large number of different ABA receptors and co-receptors within a plant species generates a complexity of heteromeric receptor complexes that has not functionally been resolved in any plant species to date. In this study, we characterized ABA receptors and co-receptors of grey poplar (Populus x canescens [Ait.] Sm.) and their capacity to regulate ABA responses. We observed a high number of regulatory combinations of holo-receptor complexes, but also some preferential and selective RCAR-PP2C interactions. Poplar and Arabidopsis ABA receptor components revealed a strong structural and functional conservation. Heterologous receptor complexes of poplar and Arabidopsis components showed functionality in vitro and regulated ABA-responsive gene expression in cells of both species. ABA-responsive promoters of Arabidopsis were also active in poplar, which was explored to generate poplar reporter lines expressing green fluorescent protein in response to ABA. The study presents a detailed analysis of receptor complexes of a tree species and shows high conservation of ABA receptor components between an annual and a perennial plant.