HCE-T cells express cornea-specific differentiation marker, PAX6 protein.
HCE-T cells express cornea-specific differentiation marker, PAX6 protein.
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HCE-T 细胞表达角膜特异性分化标记物 PAX6 蛋白。
DOI：
10.1007/s00417-022-05761-z
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发表时间：
2022
期刊：
影响因子：
--
通讯作者：
Yoshikazu Matsuoka
中科院分区：
文献类型：
--
作者：
Kaoru Araki-Sasaki;Tatsuya Kanagawa;Yoshikazu Matsuoka
图2通过RT-PCR和western blotting检测PAX6基因在HCE-T细胞中的表达情况。(a)逆转录聚合酶链反应（RT-PCR）检测HCE-T细胞中PAX6基因。以蒸馏水（DW）和从MSCs中提取的mRNA作为阴性对照。Lane M， 50-bp DNA阶梯标记。引物序列显示在下面的图中。(b) western blotting检测HCE-T细胞PAX6蛋白。以各样品中β-肌动蛋白的表达为内对照，进行RT-PCR和western blotting检测
Fig. 2 The expression of PAX6 gene in HCE-T cells by RT-PCR and western blotting.(a) PAX6 gene in HCE-T cells was detected by reverse transcription-polymerase chain reaction (RT-PCR). The distilled water (DW) and mRNA extracted from MSCs were used as the negative control. Lane M, 50-bp DNA Ladder Marker. The primer sequences were indicated in the lower panel.(b) PAX6 protein in the HCE-T cells was detected by western blotting. The expression of β-actin in each sample was used as internal control in RT-PCR and western blotting