Macleaya cordata extract alleviated oxidative stress and altered innate immune response in mice challenged with enterotoxigenic Escherichia coli
Macleaya cordata extract alleviated oxidative stress and altered innate immune response in mice challenged with enterotoxigenic Escherichia coli
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博落回提取物减轻了受到产肠毒素大肠杆菌攻击的小鼠的氧化应激并改变了先天免疫反应
DOI：
10.1007/s11427-018-9494-6
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发表时间：
2019-08-01
影响因子：
9.1
通讯作者：
Liu, Gang
中科院分区：
文献类型：
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作者：
Guan, Guiping;Ding, Sujuan;Liu, Gang
本研究探讨了膳食博落回提取物(MCE)对ICR小鼠的细菌负荷和对肠毒素大肠埃希菌(ETEC)耐药性的影响。ICR小鼠随机分为三组：(1)基础饲料组；(2)基础饲料+200 mg·kg(-1)MCE组；(3)基础饲料+ETEC组；(4)基础饲料+ETEC+200 mg·kg(-1)MCE组。在7天的预治疗后，CTRL-ETEC和MCE-ETEC小鼠被5x10(8)E.coliSec 470口服感染。结果表明，日粮中添加200 mg kg(-1)的MCE可显著降低肠道ETEC负荷(P<；0.05)，显著降低疾病相关死亡率(P<；0.05)。此外，日粮中添加MCE显著减轻了ETEC诱导的氧化应激，表现为降低了甲烷二羧醛(MDA)的丰度，提高了过氧化氢酶和谷胱甘肽过氧化物酶的活性(P<；0.05)。此外，MCE小鼠表现出较高的免疫活性，这可能进一步介导了ETEC的感染。这些结果表明，MCE对ETEC感染具有预防作用。未来的研究应该着眼于将MCE作为促进肠道健康和预防ETEC感染的一种治疗方法。
This study examines the effects of dietary Macleaya cordata extract (MCE) on bacterial burden and resistance to enterotoxigenic Escherichia coli (ETEC) in ICR mice. ICR mice were randomly distributed into one of the following groups: (i) basal diet; (ii) basal diet with 200 mg kg(-1) MCE; (iii) basal diet challenged with ETEC; and (iv) basal diet with 200 mg kg(-1) MCE and challenged with ETEC. Following a 7-day period of pre-treatment, CTRL-ETEC and MCE-ETEC mice were subjected to oral infection using 5x10(8)E. coli SEC 470. The results showed dietary 200 mg kg(-1) MCE markedly reduced intestinal ETEC burden (P < 0.05) and the disease-associated mortality was significantly alleviated in the MCE treated group (P < 0.05). In addition, dietary MCE markedly alleviated ETEC-induced oxidative stress, evidenced by the lowered methane dicarboxylic aldehyde (MDA) abundance and enhanced activities of catalase and glutathione peroxidase (P < 0.05). Furthermore, MCE mice exhibited higher immune activity, which might have further mediated ETEC infection. These results indicate MCE plays a preventative role with respect to ETEC infection. Future research should aim to develop MCE as a therapeutic approach to the promotion of intestinal health and a safeguard against ETEC infection.