Quantitative assessment of chemotropism in pollen tubes using microslit channel filters
Quantitative assessment of chemotropism in pollen tubes using microslit channel filters
复制标题
DOI：
10.1063/1.5023718
复制
发表时间：
2018-03-01
期刊：
影响因子：
3.2
通讯作者：
Higashiyama, Tetsuya
中科院分区：
文献类型：
--
作者：
Yanagisawa, Naoki;Higashiyama, Tetsuya
我们提出了一种半体外向化性测定方法，可用于评估可扩散的植物信号分子对生长的花粉管的化学吸引作用。我们在微流控装置中构建了一系列微缝隙通道，这些通道可阻止随机生长的花粉管通过，但允许对化学引诱剂有反应的花粉管通过。根据微缝隙通道的大小，80% - 100%随机生长的蓝猪耳花粉管被阻止到达引诱剂源。因此，利用该平台可以从混合群体中筛选出能够对化学引诱剂作出反应的花粉管。（C）2018作者。
We present a semi-in vitro chemotropism assay that can be used to evaluate the chemoattractant effect of diffusible plant signaling molecules on growing pollen tubes. We constructed an array of microslit channels in a microfluidic device that prevented the passage of randomly growing pollen tubes but permitted ones that are responsive to the chemoattractant. Depending on the microslit channel size, 80%-100% of the randomly growing Torenia fournieri pollen tubes were excluded from reaching the source of the attractant. Thus, the selection of pollen tubes that are capable of responding to chemoattractants from a mixed population can be realized using this platform. (C) 2018 Author(s).