Endocytosis and early endosome motility in filamentous fungi.
Endocytosis and early endosome motility in filamentous fungi.
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DOI：
10.1016/j.mib.2014.04.001
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发表时间：
2014-08
影响因子：
5.4
通讯作者：
Steinberg G
中科院分区：
文献类型：
--
作者：
Steinberg G
丝状真菌中的早期内体沿着微管移动。在几种真菌中，内体运动由驱动蛋白-3 和动力蛋白介导。在黑粉菌和曲霉中，Hook 蛋白被鉴定为内体上的运动接头。内体运动有助于分选液泡以及 mRNA 和多核糖体的分布。内体上的蛋白质翻译似乎是脓蛋白丝组装所必需的。丝状真菌的菌丝生长需要早期内体基于微管的长距离运动。自从在玉米黑粉菌中发现这一过程以来，我们对其分子基础和生物学功能的理解有了很大的进步。对玉米和构巢曲霉的研究揭示了运动蛋白驱动蛋白 3 和动力蛋白之间复杂的相互作用，它们共同支持早期内体的双向运动。基因筛选揭示了运动调节的分子机制，揭示了 Hook 蛋白作为早期内体的通用运动适配器。最近，关于内体运动的意想不到的作用的令人着迷的见解已经出现。这包括脓蛋白丝的形成和蛋白质翻译机制的细胞分布。
Early endosomes in filamentous fungi move along microtubules. In several fungi, endosome motility is mediated by kinesin-3 and dynein. In Ustilago and Aspergillus, a Hook protein was identified as a motor adapter on endosomes. Endosome motility serves sorting to the vacuole and distribution of mRNA and polysomes. Protein translation on endosomes appears to be required for septin filament assembly Hyphal growth of filamentous fungi requires microtubule-based long-distance motility of early endosomes. Since the discovery of this process in Ustilago maydis, our understanding of its molecular basis and biological function has greatly advanced. Studies in U. maydis and Aspergillus nidulans reveal a complex interplay of the motor proteins kinesin-3 and dynein, which co-operate to support bi-directional motion of early endosomes. Genetic screening has shed light on the molecular mechanisms underpinning motor regulation, revealing Hook protein as general motor adapters on early endosomes. Recently, fascinating insight into unexpected roles for endosome motility has emerged. This includes septin filament formation and cellular distribution of the machinery for protein translation.