Experimental infection and pathology of two highly pathogenic avian influenza H5N1 viruses isolated from crow and chicken in house crows (Corvus splendens)
Experimental infection and pathology of two highly pathogenic avian influenza H5N1 viruses isolated from crow and chicken in house crows (Corvus splendens)
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DOI：
10.4149/av_2020_306
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发表时间：
2020-01-01
期刊：
影响因子：
1.7
通讯作者：
Dubey, S. C.
中科院分区：
文献类型：
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作者：
Kumar, M.;Murugkar, H., V;Dubey, S. C.
研究了从乌鸦（A/crow/Assam/142119/2008）和鸡（A/chicken/锡金/151466/2009）分离的两株高致病性H5 N1禽流感病毒对家鸦（Corvus splendens）的实验感染。这两种病毒都引起了乌鸦的感染，其中六分之四和六分之三的乌鸦分别在乌鸦和鸡病毒攻击后11天内死于H5 N1感染。乌鸦的主要临床症状是翼麻痹、盘旋和斜颈。从拭子中检测到的病毒脱落在乌鸦和鸡病毒中均不持久。这两种病毒更频繁地从口腔拭子比泄殖腔拭子分离。两株病毒均从H5 N1感染后死亡的乌鸦的脑、肺、心、肝、胰、脾、大肠中分离得到。存活的禽类对H5 N1病毒感染作出血清转化反应。显微镜下，两种病毒均引起胰腺和肾脏凝固性坏死。脑组织出现胶质增生和神经元变性。这项实验研究强调，乌鸦可以感染来自不同宿主的H5 N1病毒，其致病性略有差异。因此，必须开展高致病性禽流感H5 N1病毒在与人共处的鸟类中的监测，沿着采取生物安全措施，以减少H5 N1在家禽群体中的传播。
We investigated the experimental infection of two highly pathogenic avian influenza H5N1 viruses isolated from crow (A/crow/Assam/142119/2008) and chicken (A/chicken/Sikkim/151466/2009) in house crows (Corvus splendens). Both viruses caused infection in crows, where four out of six and three out of six crows succumbed to H5N1 infection within 11 days post challenge by crow and chicken viruses, respectively. The major clinical signs in crows were wing paralysis, circling and torticollis. The virus shedding detected from swabs was not persistent in both crow nor chicken viruses. Both viruses were isolated more frequently from oral swabs than from cloacal swabs. Both virus strains were isolated from brain, lungs, heart, liver, pancreas, spleen, large intestines of crows that succumbed to H5N1 infection. The surviving birds seroconverted in response to H5N1 virus infection. Microscopically, both viruses caused coagulative necrosis in pancreas and kidneys. Brain showed gliosis and neuronal degeneration. This experimental study highlights that crows could be infected with H5N1 viruses from different hosts with minor differences in pathogenicity. Therefore, it is imperative to carry out surveillance of highly pathogenic avian influenza H5N1 virus in synanthropic birds along with biosecurity measures to mitigate the H5N1 spread in poultry population.