A New Method for Phenome Analysis of Arabidopsis thaliana :Collection of Morphological Information by Using 3-Dimensional Laser Scanner

A New Method for Phenome Analysis of Arabidopsis thaliana :Collection of Morphological Information by Using 3-Dimensional Laser Scanner
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拟南芥表型组分析的新方法:利用三维激光扫描仪采集形态信息

DOI:
10.11234/gi1990.13.595
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发表时间:
2002
期刊:
Genome Informatics
影响因子:
--
通讯作者:
M. Matsui
M. Matsui
中科院分区:
--
文献类型:
--
作者:
Tetsuro Toyoda;N. Heida;E. Kaminuma;A. Konagaya;Naoki Okamoto;M. Matsui

文献摘要

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植物基因组研究的最新进展促进了以突变体分析为主的基因功能研究的国际竞争。发现有价值的突变体在很大程度上依赖于详细标准的建立和突变体表型性状的观察。特别是对植物生长速率、收获量和种子大小等数量性状的分析,往往是多个基因功能的组合,如果没有系统的性状分析线索,单个基因的作用很容易被忽略。我们的目标是在生物信息学和3D扫描方法的帮助下建立形态特征的详细收集[2]。大约有50,000个拟南芥突变体,植物遗传学的模式生物,已经被创造出来。为了分析基因功能,需要高精度的测量技术[1]。然而,到目前为止,视觉观察已应用于大约20个定性指标,几乎没有数字符号(例如,种子大小、叶面积、叶或花的颜色)。为了使拟南芥的形态学观察与数值观测指标相适应,我们建立了三维激光扫描仪。
Recent progress in plant genome research has enhanced the international competitions for the elucidation of gene functions mainly by mutant analyses. The discovery of valuable mutants is largely relied on the establishment of detail criteria and observation of phenotypic traits of mutants. Especially for the analyses of quantitative traits (QTLs) such as plant growth rates, harvest amount and seed sizes are combination of several gene functions, and the effect of individual gene is easy to be overlooked without the clue of systematic trait analyses. Our aim is to establish detail collection of morphological traits with the help of bioinformatics and 3D scanning methods [2]. About 50,000 mutants of Arabidopsis thaliana, a model organism for plant genetics, have been created. To analyze the gene functions a high-precision measuring technique is required [1]. However, until now visual observation has been applied on about 20 qualitative indices with almost no numerical notation (e.g., seed size, leaf area, color of leaves or flowers). We constructed 3-dimentional laser scanner to fit the observation of morphology of Arabidopsis thaliana with numerical observation indices.