Sequence and regulation of a gene encoding a human 89-kilodalton heat shock protein
Sequence and regulation of a gene encoding a human 89-kilodalton heat shock protein
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DOI：
10.1128/mcb.9.6.2615-2626.1989
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发表时间：
1989-06
影响因子：
5.3
通讯作者：
E. Hickey;S. E. Brandon;G. Smale;D. Lloyd;L. Weber
中科院分区：
文献类型：
--
作者：
E. Hickey;S. E. Brandon;G. Smale;D. Lloyd;L. Weber
脊椎动物细胞合成两种形式的82到90千道尔顿的热休克蛋白，这两种蛋白由不同的基因家族编码。在HeLa细胞中，这两种蛋白质(hsp89α和hsp89β)在正常生长条件下都是丰富的，并且在热应激时合成速度加快。只有较大的形式，hsp89α，是由腺病毒E1A基因产物诱导的(M.C.Simon，K.Kitchener，H.T.Kao，E.Hickey，L.Weber，R.Voellmy，N.Heintz和J.R.Nevins，Mol.)。牢房。比奥尔。1987年7：2884-2890)。我们已经分离到一个人hsp89α基因，该基因与用作杂交探针的热诱导和E1A诱导的cDNA序列完全相同。5‘侧翼区域包含重叠和反向的共识热休克控制元件，可以在β-珠蛋白报告基因上提供热诱导表达。该基因包含10个插入序列。第一个内含子位于翻译起始密码子附近，这一排列也在果蝇hsp82基因中发现。剪接的mRNA序列含有一个开放阅读框，编码一个84,564-Dalton多肽，与其他生物的HSP82和HSP90蛋白有很高的同源性。推导出的hsp89α蛋白序列与其他文献报道的人hsp89β序列(N.F.Rebbe，J.Ware，R.M.Bertina，P.Modrich和D.W.Stafford(Gen53：235-245,1987))在732个氨基酸中至少有99个不同。在正常的细胞生长过程中，hsp89α基因的转录是由血清诱导的，但表达似乎并不局限于细胞周期的特定阶段。HSP89αm RNA比HSP70编码的m RNA更稳定，这可以解释在非应激细胞中hsp89合成的高构成比。
Vertebrate cells synthesize two forms of the 82- to 90-kilodalton heat shock protein that are encoded by distinct gene families. In HeLa cells, both proteins (hsp89 alpha and hsp89 beta) are abundant under normal growth conditions and are synthesized at increased rates in response to heat stress. Only the larger form, hsp89 alpha, is induced by the adenovirus E1A gene product (M. C. Simon, K. Kitchener, H. T. Kao, E. Hickey, L. Weber, R. Voellmy, N. Heintz, and J. R. Nevins, Mol. Cell. Biol. 7:2884-2890, 1987). We have isolated a human hsp89 alpha gene that shows complete sequence identity with heat- and E1A-inducible cDNA used as a hybridization probe. The 5'-flanking region contained overlapping and inverted consensus heat shock control elements that can confer heat-inducible expression on a beta-globin reporter gene. The gene contained 10 intervening sequences. The first intron was located adjacent to the translation start codon, an arrangement also found in the Drosophila hsp82 gene. The spliced mRNA sequence contained a single open reading frame encoding an 84,564-dalton polypeptide showing high homology with the hsp82 to hsp90 proteins of other organisms. The deduced hsp89 alpha protein sequence differed from the human hsp89 beta sequence reported elsewhere (N. F. Rebbe, J. Ware, R. M. Bertina, P. Modrich, and D. W. Stafford (Gene 53:235-245, 1987) in at least 99 out of the 732 amino acids. Transcription of the hsp89 alpha gene was induced by serum during normal cell growth, but expression did not appear to be restricted to a particular stage of the cell cycle. hsp89 alpha mRNA was considerably more stable than the mRNA encoding hsp70, which can account for the higher constitutive rate of hsp89 synthesis in unstressed cells.