Volumetry of hippocampus and amygdala with high-resolution MRI and three-dimensional analysis software: Minimizing the discrepancies between laboratories
Volumetry of hippocampus and amygdala with high-resolution MRI and three-dimensional analysis software: Minimizing the discrepancies between laboratories
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DOI：
10.1093/cercor/10.4.433
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发表时间：
2000-04-01
期刊：
影响因子：
3.7
通讯作者：
Evans, AC
中科院分区：
文献类型：
--
作者：
Pruessner, JC;Li, LM;Evans, AC
在内侧颞叶内，海马和杏仁核经常被研究人员和临床医生用于基于磁共振成像（MRI）的体积分析，然而，不同的数据采集技术、分析软件和解剖边界在过去使得难以比较来自不同实验室的MRI研究结果。为了减少这些差异，建立了一个分割协议与40名健康的正常对照受试者最近在我们的实验室扫描，数据采集进行了三维梯度回波技术，扫描进行了校正的非均匀性和注册到标准的立体定位空间分割前。使用三维软件手动进行体积分析，该软件允许同时分析矢状、冠状和水平图像。内和间评分系数产生的相关系数与其他协议。海马体积在右半球较大（3324对3208 mm（3）），而杏仁核没有半球间的差异（1154对1160 mm（3））。最重要的是，最近的分割协议海马和杏仁核的结果似乎接近对方的平均体积和半球间的差异。这表明，扫描技术的进步，体积准备和分割协议允许更精确的定义内侧颞叶结构与MRI，结果海马和杏仁核的平均体积从不同的实验室将最终变得可比。
Within the medial temporal lobe, both the hippocampus and amygdala are frequently targeted by researchers and clinicians for volumetric analysis based on magnetic resonance imaging (MRI), However, different data acquisition techniques, analysis software and anatomical boundaries have in the past made it difficult to compare results of MRI studies from different laboratories. In order to reduce these differences, a segmentation protocol was established with 40 healthy normal control subjects recently scanned in our laboratory, Data acquisition was performed with a three-dimensional gradient echo technique, and scans were corrected for non-uniformity and registered into standard stereotaxic space prior to segmentation. Volumetric analysis was performed manually using three-dimensional software that allows simultaneous analysis of sagittal, coronal and horizontal images. Intra- and inter-rater coefficients yielded correlation coefficients comparable with other protocols. The hippocampal volume was larger in the right hemisphere (3324 versus 3208 mm(3)), while no interhemispheric differences for the amygdala (1154 versus 1160 mm(3)) could be observed. Most importantly, results from recent segmentation protocols for hippocampus and amygdala seem to approach each other with regard to mean volumes and interhemispheric differences. This indicates that the advances in scanning technique, volume preparation and segmentation protocols allow a more precise definition of medial temporal lobe structures with MRI, and that results for mean volumes for hippocampus and amygdala from different laboratories will eventually become comparable.