Development of new optical imaging systems of oxygen metabolism and simultaneous measurement in hemodynamic changes using awake mice
Development of new optical imaging systems of oxygen metabolism and simultaneous measurement in hemodynamic changes using awake mice
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DOI：
10.1016/j.jneumeth.2014.08.022
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发表时间：
2014-11-30
影响因子：
3
通讯作者：
Ito, Hiroshi
中科院分区：
文献类型：
--
作者：
Takuwa, Hiroyuki;Matsuura, Tetsuya;Ito, Hiroshi
背景资料：PET可以测量人体的CBF、CBV和CMRO2，在病理诊断和临床研究中发挥重要作用。另一方面，在动物研究中，有没有光学成像系统，用于评估CBF和CBV的变化，和氧代谢，从同一个大脑区域清醒condition.New方法：在本研究中，我们开发了一个同时测量系统LSI和IOSI，这是由LDF验证。结果：LSI和LDF测量的脑血流量变化具有相关性，LSI测量的脑血流量与LDF测量的脑血流量变化具有相关性。同样，通过IOSI获得的CBV变化也与通过LDF测量的RBC浓度变化相关。FAI测得的氧代谢变化与LSI测得的CBF变化相关，尽管CBF变化大于氧代谢变化。与现有方法比较：我们发现，我们系统测得的氧代谢与CBF之间的关系与人体PET研究中CMRO2与CBF之间的关系非常一致。我们的CBF，CBV和氧代谢的测量系统不仅适用于研究神经血管耦合，而且很容易证实人体PET研究。(C)2014爱思唯尔有限公司版权所有。
Background: PET allows the measurement of CBF, CBV and CMRO2 in human and plays an important role in the diagnosis of pathologic conditions and clinical research. On the other hand, in animal studies, there is no optical imaging system for evaluating changes in CBF and CBV, and oxygen metabolism, from the same brain area under awake condition.New method: In the present study, we developed a simultaneous measurement system of LSI and IOSI, which was verified by LDF. Moreover, to evaluate oxygen metabolism, FAI was performed from the same brain area as LSI and IOSI measurements.Results: The change in CBF according to LSI was correlated with that by LDF. Similarly, the change in CBV obtained by IOSI was also correlated with RBC concentration change measured by LDF. The change in oxygen metabolism by FAI was associated with that in CBF obtained by LSI, although the change in CBF was greater than that in oxygen metabolism.Comparison with existing method(s): We revealed that the relationship between oxygen metabolism and CBF as measured by our system was in good agreement with the relationship between CMRO2 and CBF in human PET studies.Conclusions: Our measurement system of CBF, CBV and oxygen metabolism is not only useful for studying neurovascular coupling, but also easily corroborates human PET studies. (C) 2014 Elsevier B.V. All rights reserved.