Differential expression of sarcoplasmic proteins in four heterogeneous ovine skeletal muscles
Differential expression of sarcoplasmic proteins in four heterogeneous ovine skeletal muscles
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DOI：
10.1002/pmic.200600309
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发表时间：
2007-01-01
期刊：
影响因子：
3.4
通讯作者：
Laville, Elisabeth
中科院分区：
文献类型：
--
作者：
Hamelin, Muriel;Sayd, Thierry;Laville, Elisabeth
已知纤维类型分布根据肌肉功能的差异在肌肉内和肌肉之间广泛变化。采用双向电泳和MALDI-MS技术研究肌纤维类型变异的分子基础。我们比较了四种具有异质纤维型组成的羔羊骨骼肌，它们相对富含快缩纤维类型，即，半膜肌、股内侧肌、背最长肌和阔筋膜张肌。我们的结果清楚地表明，没有糖酵解代谢酶检测，包括TL是最强烈的糖酵解，使肌间分化成为可能。肌肉分化是基于参与氧化代谢的蛋白质的差异表达，不仅包括柠檬酸循环酶，而且还包括与氧化代谢、氧化应激以及可能与更高的蛋白质周转率相关的功能的其他类别的蛋白质。检测到的蛋白质参与转运（碳酸酯酶、肌红蛋白、脂肪酸结合蛋白）、错误折叠损伤的修复（热休克蛋白（HSP）60 kDa、HSP-27 kDa、α-晶状体蛋白β亚基、DJ 1、应激诱导磷蛋白）、受损蛋白质的解毒或降解（GST-Pi、醛脱氢酶、过氧化物氧还蛋白、泛素）和蛋白质合成（tRNA合成酶）。分级分离方法导致检测参与与氧化代谢相关的不同功能的蛋白质，这些蛋白质以前未显示出共存性。
Fiber-type distribution is known to vary widely within and between muscles according to differences in muscle functions. 2-DE and MALDI-MS were used to investigate the molecular basis of muscle fiber type-related variability. We compared four lamb skeletal muscles with heterogeneous fiber-type composition that are relatively rich in fast-twitch fiber types, i.e., the semimembranosus, vastus medialis, longissimus dorsi, and tensor fasciae latae (TL). Our results clearly showed that none of the glycolytic metabolism enzymes detected, including TL which was most strongly glycolytic, made intermuscular differentiation possible. Muscle differentiation was based on the differential expression of proteins involved in oxidative metabolism, including not only citric acid cycle enzymes but also other classes of proteins with functions related to oxidative metabolism, oxidative stress, and probably to higher protein turnover. Detected proteins were involved in transport (carbonate dehydratase, myoglobin, fatty acid-binding protein), repair of misfolding damage (heat shock protein (HSP) 60 kDa, HSP-27 kDa, alpha-crystallin beta subunit, DJ1, stress-induced phosphoprotein), detoxification or degradation of impaired proteins (GST-Pi, aldehyde dehydrogenase, peroxiredoxin, ubiquitin), and protein synthesis (tRNA-synthetase). The fractionating method led to the detection of proteins involved in different functions related to oxidative metabolism that have not previously been shown concomitancy.