Altered expression of heat shock proteins in embryonal carcinoma and mouse early embryonic cells
Altered expression of heat shock proteins in embryonal carcinoma and mouse early embryonic cells
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DOI：
10.1128/mcb.4.4.730-735.1984
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发表时间：
1984-04
影响因子：
5.3
通讯作者：
M. Morange;A. Diu;O. Bensaude;C. Babinet
中科院分区：
文献类型：
--
作者：
M. Morange;A. Diu;O. Bensaude;C. Babinet
在以前的论文中，我们已经表明，在没有应激的情况下，小鼠胚胎癌细胞，像小鼠早期胚胎多能细胞一样，合成高水平的89和70千道尔顿的热休克蛋白（HSP）（O。Bensaude和M. Morange，EMBO J. 2：173-177，1983）。我们在这里报告的蛋白质合成模式后，短期高温在各种小鼠胚胎癌细胞系和早期小鼠胚胎细胞。在所测试的各种细胞系中，其中两种细胞系，PCC 4-Aza R1和PCC 7-S-1009，显示出不寻常的反应，即在高温后在这些细胞中未观察到HSP合成的刺激。然而，68和105千道尔顿的HSP诱导后，可以恢复在体外分化的视黄酸引发的PCC 7-S-1009细胞。类似地，在早期小鼠胚胎中，高温不会在8细胞期诱导非组成型HSP的合成，但68千道尔顿HSP的诱导确实发生在胚泡期。这种HSP表达的转变已经在果蝇和海胆胚胎中描述过，最近在小鼠胚胎中也有描述。这可能是早期胚胎细胞的一个普遍特性。
In a previous paper, we have shown that in the absence of stress, mouse embryonal carcinoma cells, like mouse early embryo multipotent cells, synthesize high levels of 89- and 70-kilodalton heat shock proteins (HSP)(O. Bensaude and M. Morange, EMBO J. 2:173-177, 1983). We report here the pattern of proteins synthesized after a short period of hyperthermia in various mouse embryonal carcinoma cell lines and early mouse embryo cells. Among the various cell lines tested, two of them, PCC4-Aza R1 and PCC7-S-1009, showed an unusual response in that stimulation of HSP synthesis was not observed in these cells after hyperthermia. However, inducibility of 68- and 105-kilodalton HSP can be restored in PCC7-S-1009 cells after in vitro differentiation triggered by retinoic acid. Similarly, in the early mouse embryo, hyperthermia does not induce the synthesis of nonconstitutive HSP at the eight-cell stage, but induction of the 68-kilodalton HSP does occur at the blastocyst stage. Such a transition in the expression of HSP has already been described for Drosophila melanogaster and sea urchin embryos and recently for mouse embryos. It may be a general property of early embryonic cells.