Nucleotide sequence and transcriptional analysis of molecular clones of CAEV which generate infectious virus.
Nucleotide sequence and transcriptional analysis of molecular clones of CAEV which generate infectious virus.
复制标题
产生感染性病毒的 CAEV 分子克隆的核苷酸序列和转录分析。
DOI：
10.1016/0042-6822(90)90303-9
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发表时间：
1990
期刊：
影响因子：
3.7
通讯作者：
Clements,JE
中科院分区：
文献类型：
--
作者：
Saltarelli,M;Querat,G;Konings,DA;Vigne,R;Clements,JE
羊关节炎-脑炎慢病毒（CAEV）与visna病毒和其他羊慢病毒核苷酸序列同源性密切相关，与其他动物和人类慢病毒的相似性较小。CAEV的基因组组织与visna病毒和南非绵羊maedi visna病毒（SA-OMVV）以及其他灵长类慢病毒的基因组组织非常相似。CAEV基因组包括pol和env之间的小开放阅读框（ORF），这是慢病毒基因组的标志。CAEV在组织结构上最显著的区别是在环境基因上。visna病毒及其相关的SA-OMVV的Env多蛋白在翻译起始和CAEV中不存在的信号肽之间含有20个氨基酸。除核苷酸序列分析外，还采用Northern分析法确定了CAEV的转录产物。在感染克隆的细胞中存在的病毒mRNA揭示了在其他慢病毒感染中观察到的mRNA的模式特征。推测CAEV的ORF可以通过基因组定位和与visna病毒基因的氨基酸同源性进行鉴定。然而，CAEVrevgene不能以类似的方式被识别。因此，通过从感染细胞中扩增mRNA获得cDNA克隆，以确定其位置。为了确定CAEV基因组中是否含有Rev应答元件，对病毒RNA进行了二级结构分析。发现了一个稳定的茎环结构，其位置、稳定性和构型与HIV Rev应答元件相似。
The lentivirus caprine arthritis-encephalitis virus (CAEV) is closely related by nucleotide sequence homology to visna virus and other sheep lentiviruses and shows less similarity to the other animal and human lentiviruses. The genomic organization of CAEV is very similar to that of visna virus and the South African ovine maedi visna virus (SA-OMVV) as well as to those of other primate lentiviruses. The CAEV genome includes the small open reading frames (ORF) between pol and env which are the hallmarks of the lentivirus genomes. The most striking difference in the organization of CAEV is in the env gene. The Env polyproteins of visna virus and the related SA-OMVV contain 20 amino acids between the translational start and the signal peptide not present in CAEV. In addition to nucleotide sequence analysis, the transcriptional products of CAEV were determined by Northern analysis. The viral mRNA present in cells transfected with the infectious clone reveal a pattern characteristic of the mRNAs observed in other lentivirus infections. The putative tat ORF of CAEV could be identified by genomic location and amino acid homology to the visna virustatgene. However, the CAEVrevgene could not be identified in a similar fashion. Thus, to determine the location of therevORF cDNA clones were obtained by PCR amplification of the mRNA from infected cells. To determine if a Rev response element was contained in the CAEV genome, secondary structural analysis of the viral RNA was performed. A stable stem loop structure which is similar in location, stability, and configuration to that determined for the Rev response element of HIV was found.