Regulation of cytosolic and mitochondrial ATP levels in mouse eggs and zygotes
Regulation of cytosolic and mitochondrial ATP levels in mouse eggs and zygotes
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DOI：
10.1016/j.ydbio.2008.02.004
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发表时间：
2008-04-15
影响因子：
2.7
通讯作者：
Swann, Karl
中科院分区：
文献类型：
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作者：
Dumollard, Remi;Campbell, Karen;Swann, Karl
受精通过刺激过多的ATP消耗过程来激活发育，而ATP消耗过程必须通过合子中能量生产的上调来提供。精子触发的Ca（2+）振荡被认为是刺激ATP消耗和ATP供应的原因，但受精时能量产生的上调机制尚不清楚。通过测量受精卵线粒体中的[Ca(2+)]和[ATP]，我们证明精子进入触发细胞质中的Ca（2+）振荡，并将其转导为线粒体Ca（2+）振荡，从而调控线粒体ATP的产生。结果，在受精过程中，[ATP]（mito）和[ATP]（cyto）都增加了。我们还通过监测饥饿卵受精过程中的[Ca(2+)]（胞）和[ATP]（胞），观察了受精过程中ATP消耗的刺激。我们的观察结果表明，与丙酮酸相反，乳酸不促进合子中线粒体ATP的产生。因此，乳酸衍生的丙酮酸以某种方式从线粒体氧化转移到其他代谢途径。结合我们早期的研究结果，本研究证实了外源性丙酮酸在发育开始时上调ATP产生的重要作用，并表明不促进能量代谢的乳酸可能调节细胞内氧化还原电位。(C) 2008爱思唯尔公司版权所有。
Fertilization activates development by stimulating a plethora of ATP consuming processes that must be provided for by an up-regulation of energy production in the zygote. Sperm-triggered Ca(2+) oscillations are known to be responsible for the stimulation of both ATP consumption and ATP supply but the mechanism of up regulation of energy production at fertilization is still unclear. By measuring [Ca(2+)] and [ATP] in the mitochondria of fertilized mouse eggs we demonstrate that sperm entry triggers Ca(2+) oscillations in the cytosol that are transduced into mitochondrial Ca(2+) oscillations pacing mitochondrial ATP production. This results, during fertilization, in an increase in both [ATP](mito) and [ATP](cyto). We also observe the stimulation of ATP consumption accompanying fertilization by monitoring [Ca(2+)](cyto) and [ATP](cyto) during fertilization of starved eggs. Our observations reveal that lactate, in contrast to pyruvate, does not fuel mitochondrial ATP production in the zygote. Therefore lactate-derived pyruvate is somehow diverted from mitochondrial oxidation and may be channeled to other metabolic routes. Together with our earlier findings, this study confirms the essential role for exogenous pyruvate in the up-regulation of ATP production at the onset of development, and suggests that lactate, which does not fuel energetic metabolism may instead regulate the intracellular redox potential. (C) 2008 Elsevier Inc. All rights reserved.